Effect of dynamic seeding methods on the distribution of fibroblasts within human acellular dermis

• Verfasst von: Vitacolonna, Mario [VerfasserIn]
• Verfasst von: Belharazem, Djeda [VerfasserIn]
• Verfasst von: Hohenberger, Peter [VerfasserIn]
• Verfasst von: Rößner, Eric [VerfasserIn]
• Titel: Effect of dynamic seeding methods on the distribution of fibroblasts within human acellular dermis
• Verf.angabe: Mario Vitacolonna, Djeda Belharazem, Peter Hohenberger, Eric D. Roessner
• E-Jahr: 2015
• Jahr: December 2015
• Umfang: 10 S.
• Fussnoten: Gesehen am 06.12.2018
• Titel Quelle: Enthalten in: Cell and tissue banking
• Ort Quelle: Dordrecht [u.a.] : Springer Science + Business Media B.V, 2000
• Jahr Quelle: 2015
• Band/Heft Quelle: 16(2015), 4, Seite 605-614
• ISSN Quelle: 1573-6814
• DOI: doi:10.1007/s10561-015-9508-7
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cell seeding
• Sach-SW: Comparison of dynamic seeding methods
• Sach-SW: Dynamic cell seeding methods
• Sach-SW: Human acellular dermis
• Sach-SW: Scaffold seeding
• Sach-SW: Tissue-Engineering
• K10plus-PPN: 1584896566

Abstract

The purpose of this investigation was to compare different dynamic cell seeding methods regarding their seeding efficiency, homogeneity, infiltration depth and proliferation within a human acellular dermis. In addition, the growth behaviour was observed during a 12-day static in vitro culture. The dynamic methods included orbital-shaker seeding and the use of a plate centrifuge with different rotational speeds, combinations of low-pressure for matrix degassing and centrifugal seeding. Scaffolds were incubated for up to 12 days statically. Cell distribution and infiltration depth were analysed histologically at days 0, 4, 8 and 12. Seeding efficiency and cell proliferation were quantified with the MTT-assay at the same time points. Centrifugal seeding with 300g for 5 × 1 min combined with matrix degassing significantly increased the seeding efficiency and homogeneity compared to the other methods. However, following static culture, no cells were detectable after 4 days in the inner matrix zones. Furthermore, none of the degassing+centrifugation groups reached a significantly higher proliferation at day 8 compared to the reference. The use of a single dynamic method resulted in an inefficient cell seeding. We archived the highest seeding efficiency, homogeneity and infiltration depth using a combination of degassing+centrifugation at 300g for 5 × 1 min.