Surface modification of biomaterials based on high-molecular polylactic acid and their effect on inflammatory reactions of primary human monocyte-derived macrophages

• Verfasst von: Stankevich, Ksenia [VerfasserIn]
• Verfasst von: Gudima, Alexandru [VerfasserIn]
• Verfasst von: Klüter, Harald [VerfasserIn]
• Verfasst von: Kzhyshkowska, Julia [VerfasserIn]
• Titel: Surface modification of biomaterials based on high-molecular polylactic acid and their effect on inflammatory reactions of primary human monocyte-derived macrophages
• Titelzusatz: perspective for personalized therapy
• Verf.angabe: Ksenia S. Stankevich, Alexandru Gudima, Victor D. Filimonov, Harald Klüter, Evgeniya M. Mamontova, Sergei I. Tverdokhlebov, Julia Kzhyshkowska
• E-Jahr: 2015
• Jahr: 1 June 2015
• Umfang: 10 S.
• Fussnoten: Gesehen am 17.12.2018 ; Available online 26 February 2015
• Titel Quelle: Enthalten in: Materials science & engineering. C, Materials for biological applications
• Ort Quelle: Amsterdam : Elsevier, 1993
• Jahr Quelle: 2015
• Band/Heft Quelle: 51(2015), Seite 117-126
• ISSN Quelle: 1873-0191
• DOI: doi:10.1016/j.msec.2015.02.047
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Biocompatibility
• Sach-SW: Cytokines
• Sach-SW: Macrophages
• Sach-SW: Monocytes
• Sach-SW: Polylactic acid
• Sach-SW: Surface modification
• K10plus-PPN: 1585610232

Abstract

Polylactic acid (PLA) based implants can cause inflammatory complications. Macrophages are key innate immune cells that control inflammation. To provide higher biocompatibility of PLA-based implants with local innate immune cells their surface properties have to be improved. In our study surface modification technique for high-molecular PLA (MW=1,646,600g/mol) based biomaterials was originally developed and successfully applied. Optimal modification conditions were determined. Treatment of PLA films with toluene/ethanol=3/7 mixture for 10min with subsequent exposure in 0.001M brilliant green dye (BGD) solution allows to entrap approximately 10−9mol/cm2 model biomolecules. The modified PLA film surface was characterized by optical microscopy, SERS, FT-IR, UV and TG/DTA/DSC analysis. Tensile strain of modified films was determined as well. The effect of PLA films modified with BGD on the inflammatory reactions of primary human monocyte-derived macrophages was investigated. We developed in vitro test-system by differentiating primary monocyte-derived macrophages on a coating material. Type 1 and type 2 inflammatory cytokines (TNFα, CCL18) secretion and histological biomarkers (CD206, stabilin-1) expression were analyzed by ELISA and confocal microscopy respectively. BGD-modified materials have improved thermal stability and good mechanical properties. However, BGD modifications induced additional donor-specific inflammatory reactions and suppressed tolerogenic phenotype of macrophages. Therefore, our test-system successfully demonstrated specific immunomodulatory effects of original and modified PLA-based biomaterials, and can be further applied for the examination of improved coatings for implants and identification of patient-specific reactions to implants.