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Verfasst von:Hanke, Jana [VerfasserIn]   i
 Probst, Dimitri [VerfasserIn]   i
 Schwarz, Ulrich S. [VerfasserIn]   i
Titel:Dynamics of force generation by spreading platelets
Verf.angabe:Jana Hanke, Dimitri Probst, Assaf Zemel, Ulrich S. Schwarz and Sarah Köster
Umfang:11 S.
Fussnoten:Gesehen am 09.01.2019
Titel Quelle:Enthalten in: Soft matter
Jahr Quelle:2018
Band/Heft Quelle:14(2018), 31, S. 6571-6581
ISSN Quelle:1744-6848
Abstract:In order to gain more insight into the role of human platelets for blood clot formation, here we investigate the dynamics of force generation by platelet spreading onto elastic substrates of variable stiffness. Despite their small size, platelets generate high and rapidly varying traction forces on their extracellular environment, which we reconstruct with adapted implementations of Fourier transform traction cytometry. We find that while the final spread area is reached within a few minutes, the build-up of forces typically takes 10-30 minutes. In addition, we identify two distinct behaviors of individual cells, namely oscillating and non-oscillating platelets. An eigenvalue analysis of the platelet dipole tensor reveals a small anisotropy of the exerted force, which is compatible with a random distribution of a few force transmitting centers, in agreement with the observed shapes and traction patterns. We find a correlation between the maximum force level a platelet reachesand its spread area, which we explain by a thin film model for the actively contracting cell. The model reveals a large internal stress of hundreds of kPa. Experimentally we do not find any statistically relevant relation between the force level reached and the substrate stiffness within the stiffness range from 19 to 83 kPa, which might be related to the high platelet activation level used in our study. In addition, our model suggests that due to the uniquely small thickness of platelets, their mechanosensitivity might be limited to a lower stiffness range.
DOI:doi:10.1039/C8SM00895G
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Verlag: http://dx.doi.org/10.1039/C8SM00895G
 Verlag: https://pubs.rsc.org/en/content/articlelanding/2018/sm/c8sm00895g
 DOI: https://doi.org/10.1039/C8SM00895G
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1586053868
Verknüpfungen:→ Zeitschrift

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