| Online-Ressource |
Verfasst von: | Kitanovic, Ana [VerfasserIn]  |
| Bonowski, Felix [VerfasserIn]  |
| Heigwer, Florian [VerfasserIn]  |
| Kitanovic, Igor [VerfasserIn]  |
| Wölfl, Stefan [VerfasserIn]  |
Titel: | Acetic acid treatment in S. cerevisiae creates significant energy deficiency and nutrient starvation that is dependent on the activity of the mitochondrial transcriptional complex Hap2-3-4-5 |
Verf.angabe: | Ana Kitanovic, Felix Bonowski, Florian Heigwer, Peter Ruoff, Igor Kitanovic, Christin Ungewiss, Stefan Wölfl |
E-Jahr: | 2012 |
Jahr: | September 2012 |
Umfang: | 12 S. |
Fussnoten: | Gesehen am 04.02.2019 |
Titel Quelle: | Enthalten in: Frontiers in oncology |
Ort Quelle: | Lausanne : Frontiers Media, 2011 |
Jahr Quelle: | 2012 |
Band/Heft Quelle: | 2(2012), Artikel-ID 118, Seite 1-12 |
ISSN Quelle: | 2234-943X |
Abstract: | Metabolic pathways play an indispensable role in supplying cellular systems with energy and molecular building blocks for growth, maintenance and repair and are tightly linked with lifespan and systems stability of cells. For optimal growth and survival cells rapidly adopt to environmental changes. Accumulation of acetic acid in stationary phase budding yeast cultures is considered to be a primary mechanism of chronological aging and induction of apoptosis in yeast, which has prompted us to investigate the dependence of acetic acid toxicity on extracellular conditions in a systematic manner. Using an automated computer controlled assay system, we investigated and model the dynamic interconnection of biomass yield- and growth rate-dependence on extracellular glucose concentration, pH conditions and acetic acid concentration. Our results show that toxic concentrations of acetic acid inhibit glucose consumption and reduce ethanol production. In absence of carbohydrates uptake, cells initiate synthesis of storage carbohydrates, trehalose and glycogen, and upregulate gluconeogenesis. Accumulation of trehalose and glycogen, and induction of gluconeogenesis depends on mitochondrial activity, investigated by depletion of the Hap2-3-4-5 complex. Analyzing the activity of glycolytic enzymes, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), pyruvate kinase (PYK) and glucose-6-phosphate dehydrogenase (G6PDH) we found that while high acetic acid concentration increased their activity, lower acetic acids concentrations significantly inhibited these enzymes. With this study we determined growth and functional adjustment of metabolism to acetic acid accumulation in a complex range of extracellular conditions. Our results show that substantial acidification of the intracellular environment, resulting from accumulation of dissociated acetic acid in the cytosol, is required for acetic acid toxicity, which creates a state of energy deficiency and nutrient starvation. |
DOI: | doi:10.3389/fonc.2012.00118 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.3389/fonc.2012.00118 |
| Volltext: https://www.frontiersin.org/articles/10.3389/fonc.2012.00118/full |
| DOI: https://doi.org/10.3389/fonc.2012.00118 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Acetic Acid |
| growth dynamic |
| Hap4p |
| intracellular pH homeostasis |
| metabolic control |
| response surface modelling |
K10plus-PPN: | 1587203707 |
Verknüpfungen: | → Zeitschrift |
Acetic acid treatment in S. cerevisiae creates significant energy deficiency and nutrient starvation that is dependent on the activity of the mitochondrial transcriptional complex Hap2-3-4-5 / Kitanovic, Ana [VerfasserIn]; September 2012 (Online-Ressource)