Status: Bibliographieeintrag
Standort: ---
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| Online-Ressource |
Verfasst von: | Sebens, Susanne [VerfasserIn]  |
| Schirrmacher, Volker [VerfasserIn]  |
| Umansky, Viktor [VerfasserIn]  |
Titel: | Sialoadhesin-positive host macrophages play an essential role in graft-versus-leukemia reactivity in mice |
Verf.angabe: | Susanne Müerköster, Marian Rocha, Paul R. Crocker, Volker Schirrmacher, and Victor Umansky |
E-Jahr: | 1999 |
Jahr: | June 15, 1999 |
Umfang: | 12 S. |
Fussnoten: | Gesehen am 20.02.2019 ; Der Vorname von Viktor Umansky ist in der Vorlage mit "c" geschrieben |
Titel Quelle: | Enthalten in: Blood |
Ort Quelle: | Washington, DC : American Society of Hematology, 1946 |
Jahr Quelle: | 1999 |
Band/Heft Quelle: | 93(1999), 12, Seite 4375-4386 |
ISSN Quelle: | 1528-0020 |
Abstract: | We recently established an effective immune T-cell-mediated graft-versus-leukemia (GVL) murine model system in which complete tumor remissions were achievable even in advanced metastasized cancer. We now describe that this T-cell-mediated therapy is dependent on host macrophages expressing the lymphocyte adhesion molecule sialoadhesin (Sn). Depletion of Kupffer cells in tumor-bearing mice during adoptive immunotherapy (ADI) or the treatment of these animals with anti-Sn monoclonal antibodies led to complete or partial inhibition of the immune T-cell-mediated therapeutic effect. Furthermore, Sn+ host macrophages in livers formed clusters during ADI with donor CD8 T cells. To test for a possible antigen presentation function of these macrophages, we used as an in vitro model the antigen β-galactosidase for which a dominant major histocompatibility complex (MHC) class I Ld-restricted peptide epitope is known to be recognized by specific CD8 cytotoxic T lymphocytes (CTL). We demonstrate that purified Sn+ macrophages can process exogenous β-galactosidase and stimulate MHC class I peptide-restricted CTL responses. Thus, Sn+ macrophages, which are significantly increased in the liver after ADI, may process tumor-derived proteins via the MHC class I pathway as well as via the MHC class II pathway, as shown previously, and present respective peptide epitopes to CD8 as well as to CD4 immune T cells, respectively. The synergistic interactions observed before between immune CD4 and CD8 T cells during ADI could thus occur in the observed clusters with Sn+ host macrophages. |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://www.bloodjournal.org/content/93/12/4375 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1587840987 |
Verknüpfungen: | → Zeitschrift |
Sialoadhesin-positive host macrophages play an essential role in graft-versus-leukemia reactivity in mice / Sebens, Susanne [VerfasserIn]; June 15, 1999 (Online-Ressource)
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