Increased expression of epidermal fatty acid binding protein, cofilin, and 14-3-3-[sigma] (stratifin) detected by two-dimensional gel electrophoresis, mass spectrometry and microsequencing of drug-resistant human adenocarcinoma of the pancreas

• Verfasst von: Sinha-von Jaschke, Pranav [VerfasserIn]
• Verfasst von: Schadendorf, Dirk [VerfasserIn]
• Titel: Increased expression of epidermal fatty acid binding protein, cofilin, and 14-3-3-[sigma] (stratifin) detected by two-dimensional gel electrophoresis, mass spectrometry and microsequencing of drug-resistant human adenocarcinoma of the pancreas
• Verf.angabe: Pranav Sinha, Gero Hütter, Eckart Köttgen, Manfred Dietel, Dirk Schadendorf, Hermann Lage
• Umfang: 9 S.
• Fussnoten: Gesehen am 21.02.2019 ; Im Titel ist [sigma] als griechischer Buchstabe dargestellt.
• Titel Quelle: Enthalten in: Electrophoresis
• Jahr Quelle: 1999
• Band/Heft Quelle: 20(1999), 14, S. 2952-2960
• ISSN Quelle: 1522-2683
• DOI: doi:10.1002/(SICI)1522-2683(19991001)20:14<2952¬AID-ELPS2952>3.0.CO;2-H
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1587881098

Abstract

In order to study possible mechanisms leading to chemoresistance in pancreatic adenocarcinoma we examined the global protein expression of pancreatic cancer cells in vitro. We used a cell culture model derived from the adenocarcinoma of the pancreas (EPP85-181P). A classical multidrug-resistant subline, EPP85-181RDB, selected in presence of daunorubicin, and an atypical multidrug-resistant cell variant, EPP85-181RNOV, selected in presence of mitoxantrone, were analyzed using two-dimensional electrophoresis. After staining and image analysis, spots of interest were isolated using preparative two-dimensional electrophoresis and subjected to mass spectrometry and microsequencing. Three proteins, E-FABP, cofilin, and 14-3-3-σ (stratifin), were overexpressed in chemoresistant cell lines. Cofilin was present in both multidrug in chemoresistant cell lines. Cofilin was present in both multidrug-resistant cell lines. E-FABP and 14-3-3-σ (stratifin) was found to be overexpressed onlyin the mitoxantrone-selected atypical multidrug-resistant cell line. The possible significance of these findings is discussed.