Identification of novel proteins associated with the development of chemoresistance in malignant melanoma using two-dimensional electrophoresis

• Verfasst von: Sinha-von Jaschke, Pranav [VerfasserIn]
• Verfasst von: Kern, Monika [VerfasserIn]
• Verfasst von: Schnölzer, Martina [VerfasserIn]
• Verfasst von: Schadendorf, Dirk [VerfasserIn]
• Titel: Identification of novel proteins associated with the development of chemoresistance in malignant melanoma using two-dimensional electrophoresis
• Verf.angabe: Pranav Sinha, Sandra Kohl, Jochen Fischer, Gero Hütter, Monika Kern, Eckard Köttgen, Manfred Dietel, Hermann Lage, Martina Schnölzer, Dirk Schadendorf
• E-Jahr: 2000
• Jahr: 24 August 2000
• Umfang: 10 S.
• Fussnoten: Gesehen am 26.02.2019
• Titel Quelle: Enthalten in: Electrophoresis
• Ort Quelle: Weinheim : Wiley-Blackwell, 1980
• Jahr Quelle: 2000
• Band/Heft Quelle: 21(2000), 14, Seite 3048-3057
• ISSN Quelle: 1522-2683
• DOI: doi:10.1002/1522-2683(20000801)21:14<3048::AID-ELPS3048>3.0.CO;2-W
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Chemoresistance
• Sach-SW: Melanoma cells
• Sach-SW: Two-dimensional electrophoresis
• K10plus-PPN: 1588048225

Abstract

A model system for studying chemoresistance in human melanoma cells (MeWo) has been established utilizing the four commonly used cytotoxic drugs vindesine, cisplatin, fotemustine and etoposide to yield stable drug-resistant sublines. We analyzed phenotypical differences between MeWo cells and their chemoresistant counterparts using two-dimensional electrophoresis. Proteins that were overexpressed in chemoresistant cell lines were purified and identified using matrix assisted laser desorption/ionization-time of flight — mass spectrometry (MALDI-TOF-MS) and microsequencing. Here we show that four proteins, namely the translationally controlled tumor protein, the human elongation factor 1-δ, tetratricopeptide repeat protein and the isoform 14-3-3-γ of the 14-3-3-family are overexpressed in chemoresistant melanoma cell lines. The significance of these findings is now being verified using transfection experiments with the aim of developing more effective chemotherapy protocols.