Protein labeling with the labeling precursor [18F]SiFA-SH for positron emission tomography

• Verfasst von: Wängler, Björn [VerfasserIn]
• Verfasst von: Niedermoser, Sabrina [VerfasserIn]
• Verfasst von: Wängler, Carmen [VerfasserIn]
• Titel: Protein labeling with the labeling precursor [18F]SiFA-SH for positron emission tomography
• Verf.angabe: Björn Wängler, Alexey P. Kostikov, Sabrina Niedermoser, Joshua Chin, Katy Orchowski, Esther Schirrmacher, Liuba Iovkova-Berends, Klaus Jurkschat, Carmen Wängler, Ralf Schirrmacher
• E-Jahr: 2012
• Jahr: 04 October 2012
• Umfang: 6 S.
• Fussnoten: Im Titel ist die Zahl 18 hochgestellt ; Gesehen am 27.02.2019
• Titel Quelle: Enthalten in: Nature protocols
• Ort Quelle: Basingstoke : Nature Publishing Group, 2006
• Jahr Quelle: 2012
• Band/Heft Quelle: 7(2012), 11, Seite 1964-1969
• ISSN Quelle: 1750-2799
• DOI: doi:10.1038/nprot.2012.111
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1588148726

Abstract

Proteins previously derivatized with the cross-coupling reagent sulfo-SMCC (4-(N-maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxy-succinimide ester sodium salt) can be easily labeled in high radiochemical yields with the silicon-fluoride acceptor (SiFA) reagent [18F]SiFA-SH, obtained via isotopic exchange, by thiol-maleimide coupling chemistry (n = 10). The specific activity of SiFA-SH obtained in a one-step labeling reaction was >18.5 GBq μmol−1 (>500 Ci mmol−1). The number of SiFA building blocks per protein molecule is defined by the previously introduced number of maleimide groups, which can be determined by a simple and convenient Ellman's assay. Not more than two maleimide groups are introduced using sulfo-SMCC, thereby keeping the modification of the protein low and preserving its biological activity.