From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin

• Verfasst von: Huck, Volker [VerfasserIn]
• Verfasst von: Gorzelanny, Christian [VerfasserIn]
• Verfasst von: Thorn, Verena [VerfasserIn]
• Verfasst von: Meß, Christian [VerfasserIn]
• Verfasst von: Schneider, Stefan W. [VerfasserIn]
• Titel: From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin
• Verf.angabe: Volker Huck, Christian Gorzelanny, Kai Thomas, Valentina Getova, Verena Niemeyer, Katharina Zens, Tim R. Unnerstall, Julia S. Feger, Mohammad A. Fallah, Dieter Metze, Sonja Ständer, Thomas A. Luger, Karsten Koenig, Christian Mess and Stefan W. Schneider
• E-Jahr: 2016
• Jahr: 23 March 2016
• Umfang: 12 S.
• Fussnoten: Gesehen am 27.02.2019
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Springer Nature, 2011
• Jahr Quelle: 2016
• Band/Heft Quelle: 6(2016) Artikel-Nummer 22789, 12 Seiten
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/srep22789
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1588183602

Abstract

The application of multiphoton microscopy in the field of biomedical research and advanced diagnostics promises unique insights into the pathophysiology of inflammatory skin diseases. In the present study, we combined multiphoton-based intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within the scope of a clinical trial of atopic dermatitis with the aim of providing personalised data on the aetiopathology of inflammation in a non-invasive manner at patients’ bedsides. These ‘optical biopsies’ generated via MPT were morphologically analysed and aligned with classical skin histology. Because of its subcellular resolution, MPT provided evidence of a redistribution of mitochondria in keratinocytes, indicating an altered cellular metabolism. Two independent morphometric algorithms reliably showed an even distribution in healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using MPT-FLIM, detection of the onset and progression of inflammatory processes could be achieved. In conclusion, the change in the distribution of mitochondria upon inflammation and the verification of an altered cellular metabolism facilitate a better understanding of inflammatory skin diseases and may permit early diagnosis and therapy.