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Verfasst von:Weber, Laura [VerfasserIn]   i
 Isse, Awale [VerfasserIn]   i
 Löffler, Felix [VerfasserIn]   i
Titel:Antibody fingerprints in lyme disease deciphered with high density peptide arrays
Verf.angabe:Laura K. Weber, Awale Isse, Simone Rentschler, Richard E. Kneusel, Andrea Palermo, Jürgen Hubbuch, Alexander Nesterov‐Mueller, Frank Breitling, Felix F. Loeffler
E-Jahr:2017
Jahr:21 June 2017
Umfang:10 S.
Fussnoten:Gesehen am 28.03.2019
Titel Quelle:Enthalten in: Engineering in life sciences
Ort Quelle:Weinheim : Wiley-VCH, 2001
Jahr Quelle:2017
Band/Heft Quelle:17(2017), 10, Seite 1078-1087
ISSN Quelle:1618-2863
Abstract:Lyme disease is the most common tick-borne infectious disease in Europe and North America. Previous studies discovered the immunogenic role of a surface-exposed lipoprotein (VlsE) of Borreliella burgdorferi. We employed high density peptide arrays to investigate the antibody response to the VlsE protein in VlsE-positive patients by mapping the protein as overlapping peptides and subsequent in-depth epitope substitution analyses. These investigations led to the identification of antibody fingerprints represented by a number of key residues that are indispensable for the binding of the respective antibody. This approach allows us to compare the antibody specificities of different patients to the resolution of single amino acids. Our study revealed that the sera of VlsE-positive patients recognize different epitopes on the protein. Remarkably, in those cases where the same epitope is targeted, the antibody fingerprint is almost identical. Furthermore, we could correlate two fingerprints with human autoantigens and an Epstein-Barr virus epitope; yet, the link to autoimmune disorders seems unlikely and must be investigated in further studies. The other three fingerprints are much more specific for B. burgdorferi. Since antibody fingerprints of longer sequences have proven to be highly disease specific, our findings suggest that the fingerprints could function as diagnostic markers that can reduce false positive test results.
DOI:doi:10.1002/elsc.201700062
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1002/elsc.201700062
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/elsc.201700062
 DOI: https://doi.org/10.1002/elsc.201700062
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Antibody specificity
 B. burgdorferi
 Etiology
 Substitution analysis
 Variable surface protein
K10plus-PPN:1662474385
Verknüpfungen:→ Zeitschrift

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