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Verfasst von:Wortmann, Markus [VerfasserIn]   i
 Peters, Andreas [VerfasserIn]   i
 Hakimi, Maani [VerfasserIn]   i
 Böckler, Dittmar [VerfasserIn]   i
 Dihlmann, Susanne [VerfasserIn]   i
Titel:Necrotic cell debris induces a NF-κB-driven inflammasome response in vascular smooth muscle cells derived from abdominal aortic aneurysms (AAA-SMC)
Verf.angabe:Markus Wortmann, Ekaterina Skorubskaya, Andreas S. Peters, Maani Hakimi, Dittmar Böckler, Susanne Dihlmann
E-Jahr:2019
Jahr:16 February 2019
Umfang:7 S.
Fussnoten:Gesehen am 29.03.2019
Titel Quelle:Enthalten in: Biochemical and biophysical research communications
Ort Quelle:Orlando, Fla. : Academic Press, 1959
Jahr Quelle:2019
Band/Heft Quelle:511(2019), 2, Seite 343-349
ISSN Quelle:1090-2104
Abstract:Abdominal aortic aneurysm (AAA) is a multi-factorial progressive vascular disease with life-threatening complications. Increasing evidence suggests that smooth muscle cell (SMC) dysfunction and cell death contribute to dilatation and rupture of the aorta by inducing an inflammatory response. The exact mechanism of this response however, is incompletely understood. We here investigated in vitro the capacity of autologous necrotic cell debris (CD) to induce inflammasome components and inflammatory mediators in aortic SMC (AAA-SMC) isolated from patients with AAA undergoing surgical repair. AAA-SMCs were additionally primed with Interferon- γ (IFN-γ) before treatment with CD in order to mimic the proinflammatory status caused by higher IFN-γ concentrations that have been demonstrated in the wall of AAAs. Real-time RT-PCR revealed that CD significantly increased NLRP3 and IL1B mRNA expression in different SMC cultures within 6h of exposure. Priming of the AAA-SMC with IFN-γ significantly increased expression of NLRP3, AIM2, IFI16 and CASP1 mRNAs, whereas IL1B mRNA was reduced. Additional exposure of IFN-γ-primed AAA-SMC to CD for 6-24h, further augmented expression of AIM2, NLRP3, and Caspase-1 protein levels. Analysis of the SMC supernatants by ELISA revealed CD-induced release of the senescence-associated cytokines IL-6 and MCP-1 in native and IFN-γ-primed SMC, whereas no secretion of Interleukin-(IL) 1α and IL-1β secretion were observed. Our results implicate a role of necrotic cell debris derived from dead neighboring cells in SMC dysfunction and in inflammatory response of AAA tissue.
DOI:doi:10.1016/j.bbrc.2019.02.051
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.bbrc.2019.02.051
 Volltext: http://www.sciencedirect.com/science/article/pii/S0006291X19302384
 DOI: https://doi.org/10.1016/j.bbrc.2019.02.051
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Abdominal aortic aneurysm
 AIM2
 Inflammasome
 Necrosis
 NLRP3
 VSMC
K10plus-PPN:1662537182
Verknüpfungen:→ Zeitschrift

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