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Verfasst von:Wabnitz, Guido H. [VerfasserIn]   i
 Kirchgessner, Henning [VerfasserIn]   i
 Jahraus, Beate [VerfasserIn]   i
 Umansky, Ludmila [VerfasserIn]   i
 Samstag, Yvonne [VerfasserIn]   i
Titel:Protein phosphatase 1α and cofilin regulate nuclear translocation of NF-[kappa]B and promote expression of the anti-inflammatory cytokine interleukin-10 by T cells
Verf.angabe:Guido H. Wabnitz, Henning Kirchgessner, Beate Jahraus, Ludmila Umansky, Shirish Shenolikar, Yvonne Samstag
E-Jahr:2018
Jahr:4 September 2018
Umfang:17 S.
Fussnoten:Gesehen am 11.04.2019
Titel Quelle:Enthalten in: Molecular and cellular biology
Ort Quelle:Washington, DC : Soc., 1981
Jahr Quelle:2018
Band/Heft Quelle:38(2018,17) Artikel-Nummer e00041-18, 17 Seiten
ISSN Quelle:1098-5549
Abstract:While several protein serine/threonine kinases control cytokine production by T cells, the roles of serine/threonine phosphatases are largely unexplored. Here, we analyzed the involvement of protein phosphatase 1α (PP1α) in cytokine synthesis following costimulation of primary human T cells. Small interfering RNA (siRNA)-mediated knockdown of PP1α (PP1KD) or expression of a dominant negative PP1α (D95N-PP1) drastically diminished interleukin-10 (IL-10) production. Focusing on a key transcriptional activator of human IL-10, we demonstrate that nuclear translocation of NF-κB was significantly inhibited in PP1KD or D95N-PP1 cells. Interestingly, knockdown of cofilin, a known substrate of PP1 containing a nuclear localization signal, also prevented nuclear accumulation of NF-κB. Expression of a constitutively active nonphosphorylatable S3A-cofilin in D95N-PP1 cells restored nuclear translocation of NF-κB and IL-10 expression. Subpopulation analysis revealed that defective nuclear translocation of NF-κB was most prominent in CD4+ CD45RA- CXCR3- T cells that included IL-10-producing TH2 cells. Together these findings reveal novel functions for PP1α and its substrate cofilin in T cells namely the regulation of the nuclear translocation of NF-κB and promotion of IL-10 production. These data suggest that stimulation of PP1α could limit the overwhelming immune responses seen in chronic inflammatory diseases.
DOI:doi:10.1128/MCB.00041-18
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1128/MCB.00041-18
 DOI: https://doi.org/10.1128/MCB.00041-18
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:cytokines
 phosphatase
 T-cell activation
K10plus-PPN:1663120196
Verknüpfungen:→ Zeitschrift

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