Clinical applications of MS-based protein quantification

• Verfasst von: Sabbagh, Bassel [VerfasserIn]
• Verfasst von: Mindt, Sonani [VerfasserIn]
• Verfasst von: Neumaier, Michael [VerfasserIn]
• Verfasst von: Findeisen, Peter [VerfasserIn]
• Titel: Clinical applications of MS-based protein quantification
• Verf.angabe: Bassel Sabbagh, Sonani Mindt, Michael Neumaier, Peter Findeisen
• E-Jahr: 2016
• Jahr: 06 January 2016
• Umfang: 23 S.
• Fussnoten: Gesehen am 23.04.2019
• Titel Quelle: Enthalten in: Proteomics. Clinical applications
• Ort Quelle: Weinheim : Wiley VCH, 2007
• Jahr Quelle: 2016
• Band/Heft Quelle: 10(2016), 4, Seite 323-345
• ISSN Quelle: 1862-8354
• DOI: doi:10.1002/prca.201500116
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Mass spectrometry
• Sach-SW: Protein quantification
• K10plus-PPN: 1663468087

Abstract

Mass spectrometry-based assays are increasingly important in clinical laboratory medicine and nowadays are already commonly used in several areas of routine diagnostics. These include therapeutic drug monitoring, toxicology, endocrinology, pediatrics, and microbiology. Accordingly, some of the most common analyses are therapeutic drug monitoring of immunosuppressants, vitamin D, steroids, newborn screening, and bacterial identification. However, MS-based quantification of peptides and proteins for routine diagnostic use is rather rare up to now despite excellent analytical specificity and good sensitivity. Here, we want to give an overview over current fit-for-purpose assays for MS-based protein quantification. Advantages as well as challenges of this approach will be discussed with focus on feasibility for routine diagnostic use.