| |  Online-Ressource |
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| Verfasst von: | Yücel, Sara Suna [VerfasserIn]  |
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| | Lorenzoni, Alessandra [VerfasserIn] |
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| | Lemberg, Marius [VerfasserIn] |
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| Titel: | The Metastable XBP1u transmembrane domain defines determinants for intramembrane proteolysis by signal peptide peptidase |
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| Verf.angabe: | Sara Suna Yücel, Walter Stelzer, Alessandra Lorenzoni, Manfred Wozny, Dieter Langosch, and Marius K. Lemberg |
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| E-Jahr: | 2019 |
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| Jahr: | March 12, 2019 |
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| Umfang: | 25 S. |
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| Fussnoten: | Gesehen am 24.04.2019 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 26(2019), 11, Seite 3087-3099, e1-e11 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | Summary - Unspliced XBP1 mRNA encodes XBP1u, the transcriptionally inert variant of the unfolded protein response (UPR) transcription factor XBP1s. XBP1u targets its mRNA-ribosome-nascent-chain-complex to the endoplasmic reticulum (ER) to facilitate UPR activation and prevents overactivation. Yet, its membrane association is controversial. Here, we use cell-free translocation and cellular assays to define a moderately hydrophobic stretch in XBP1u that is sufficient to mediate insertion into the ER membrane. Mutagenesis of this transmembrane (TM) region reveals residues that facilitate XBP1u turnover by an ER-associated degradation route that is dependent on signal peptide peptidase (SPP). Furthermore, the impact of these mutations on TM helix dynamics was assessed by residue-specific amide exchange kinetics, evaluated by a semi-automated algorithm. Based on our results, we suggest that SPP-catalyzed intramembrane proteolysis of TM helices is not only determined by their conformational flexibility, but also by side-chain interactions near the scissile peptide bond with the enzyme’s active site. |
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| DOI: | doi:10.1016/j.celrep.2019.02.057 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.celrep.2019.02.057 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S2211124719302360 |
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| | DOI: https://doi.org/10.1016/j.celrep.2019.02.057 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | ERAD |
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| | exosite |
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| | GxGD aspartic intramembrane protease |
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| | HO1 |
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| | regulated intramembrane proteolysis |
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| | SPP |
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| | subsite |
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| | transmembrane helix dynamics |
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| | XBP1u |
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| K10plus-PPN: | 1663523169 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ Metastable XBP1u transmembrane domain defines determinants for intramembrane proteolysis by signal peptide peptidase / Yücel, Sara Suna [VerfasserIn]; March 12, 2019 (Online-Ressource)
