Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Ratliff, Miriam [VerfasserIn]   i
Titel:MIB-1 is required for spermatogenesis and facilitates LIN-12 and GLP-1 activity in Caenorhabditis elegans
Verf.angabe:Miriam Ratliff, Katherine L. Hill-Harfe, Elizabeth J. Gleason, Huiping Ling, Tim L. Kroft, and Steven W. L’Hernault
E-Jahr:2018
Jahr:May 1, 2018
Umfang:21 S.
Fussnoten:Gesehen am 29.04.2019
Titel Quelle:Enthalten in: Genetics
Ort Quelle:Oxford : Oxford University Press, 1916
Jahr Quelle:2018
Band/Heft Quelle:209(2018), 1, Seite 173-193
ISSN Quelle:1943-2631
Abstract:Covalent attachment of ubiquitin to substrate proteins changes their function or marks them for proteolysis, and the specificity of ubiquitin attachment is mediated by the numerous E3 ligases encoded by animals. Mind Bomb is an essential E3 ligase during Notch pathway signaling in insects and vertebrates. While Caenorhabditis elegans encodes a Mind Bomb homolog (mib-1), it has never been recovered in the extensive Notch suppressor/enhancer screens that have identified numerous pathway components. Here, we show that C. elegans mib-1 null mutants have a spermatogenesis-defective phenotype that results in a heterogeneous mixture of arrested spermatocytes, defective spermatids, and motility-impaired spermatozoa. mib-1 mutants also have chromosome segregation defects during meiosis, molecular null mutants are intrinsically temperature-sensitive, and many mib-1 spermatids contain large amounts of tubulin. These phenotypic features are similar to the endogenous RNA intereference (RNAi) mutants, but mib-1 mutants do not affect RNAi. MIB-1 protein is expressed throughout the germ line with peak expression in spermatocytes followed by segregation into the residual body during spermatid formation. C. elegans mib-1 expression, while upregulated during spermatogenesis, also occurs somatically, including in vulva precursor cells. Here, we show that mib-1 mutants suppress both lin-12 and glp-1 (C. elegans Notch) gain-of-function mutants, restoring anchor cell formation and a functional vulva to the former and partly restoring oocyte production to the latter. However, suppressed hermaphrodites are only observed when grown at 25°, and they are self-sterile. This probably explains why mib-1 was not previously recovered as a Notch pathway component in suppressor/enhancer selection experiments.
DOI:doi:10.1534/genetics.118.300807
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1534/genetics.118.300807
 Volltext: http://www.genetics.org/content/209/1/173
 DOI: https://doi.org/10.1534/genetics.118.300807
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:C. elegans
 Mind Bomb
 Notch signaling
 spermatogenesis
 ubiquitin E3 ligase
K10plus-PPN:1663750130
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68383402   QR-Code
zum Seitenanfang