Mastering complexity

• Verfasst von: Göpfrich, Kerstin [VerfasserIn]
• Verfasst von: Platzman, Ilia [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Mastering complexity
• Titelzusatz: towards bottom-up construction of multifunctional eukaryotic synthetic cells
• Verf.angabe: Kerstin Göpfrich, Ilia Platzman, and Joachim P. Spatz
• E-Jahr: 2018
• Jahr: 21 April 2018
• Umfang: 14 S.
• Fussnoten: Gesehen am 08.05.2019
• Titel Quelle: Enthalten in: Trends in biotechnology
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1983
• Jahr Quelle: 2018
• Band/Heft Quelle: 36(2018), 9, Seite 938-951
• ISSN Quelle: 1879-3096
• DOI: doi:10.1016/j.tibtech.2018.03.008
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: bottom-up synthetic biology
• Sach-SW: compartment
• Sach-SW: DNA nanotechnology
• Sach-SW: droplet-based microfluidics
• Sach-SW: giant unilamellar vesicle
• Sach-SW: synthetic cell
• K10plus-PPN: 1664973559

Abstract

With the ultimate aim to construct a living cell, bottom-up synthetic biology strives to reconstitute cellular phenomena in vitro - disentangled from the complex environment of a cell. Recent work towards this ambitious goal has provided new insights into the mechanisms governing life. With the fast-growing library of functional modules for synthetic cells, their classification and integration become increasingly important. We discuss strategies to reverse-engineer and recombine functional parts for synthetic eukaryotes, mimicking the characteristics of nature’s own prototype. Particularly, we focus on large outer compartments, complex endomembrane systems with organelles, and versatile cytoskeletons as hallmarks of eukaryotic life. Moreover, we identify microfluidics and DNA nanotechnology as two technologies that can integrate these functional modules into sophisticated multifunctional synthetic cells.