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Titel:Photoswitching Proteins
Titelzusatz:methods and protocols
Mitwirkende:Cambridge, Sidney [Hrsg.]   i
Verf.angabe:edited by Sidney Cambridge
Verlagsort:New York, NY
Verlag:Humana Press
Jahr:2014
Umfang:Online-Ressource (X, 278 p. 58 illus., 53 illus. in color, online resource)
Gesamttitel/Reihe:Methods in molecular biology ; 1148
 Springer eBook Collection
 SpringerLink : Bücher
ISBN:978-1-4939-0470-9
Abstract:Photoinduced Damage Resulting from Fluorescence Imaging of Live Cells -- Modification of Purified Proteins with Photochemical Protection Compounds for High-Resolution Photoactivation of Protein Function In Vitro and In Vivo -- Optochemical Activation of Kinase Function in Live Cells -- Photoswitching of Cell Surface Receptors Using Tethered Ligands -- Photocontrol of AMPA Receptors with a Photochromic Ligand -- Photoconversion of CFP to Study Neuronal Tissue with Electron Microscopy -- Light-Inducible Gene Regulation with Engineered Zinc Finger Proteins -- Manipulation of Plasma Membrane Phosphoinositides using Photoinduced Protein-protein Interactions -- A Roadmap to Applying Optogenetics in Neuroscience -- Salvaging Ruins: Reverting Blind Retinas into Functional Visual Sensors -- Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity -- Structural Basis of Photoswitching in Fluorescent Proteins -- Using Photoactivatable GFP to Track Axonal Transport Kinetics -- In Vivo Cell Tracking Using PhOTO Zebrafish -- In vivo Optogenetics for Light-induced Oxidative Stress in Transgenic Zebrafish Expressing the KillerRed Photosensitizer Protein -- Photoactivatable Fluorescent Proteins for Super-Resolution Microscopy -- pcSOFI as a Smart Label-based Superresolution Microscopy Technique.
 Photoswitching Proteins: Methods and Protocols, focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems.
DOI:doi:10.1007/978-1-4939-0470-9
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Resolving-System: https://doi.org/10.1007/978-1-4939-0470-9
 Cover: https://swbplus.bsz-bw.de/bsz405483074cov.jpg
 DOI: https://doi.org/10.1007/978-1-4939-0470-9
Schlagwörter:(s)Proteine   i / (s)Fotochromie   i
Datenträger:Online-Ressource
Sprache:eng
Reproduktion:Druckausg.: Photoswitching proteins. - New York, NY: Humana Press, 2014. - X, 278 S
RVK-Notation:WC 4150   i
K10plus-PPN:1656302519
Verknüpfungen:→ Übergeordnete Aufnahme, Artikel

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