Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications

• Verfasst von: Tardajos, Myriam G. [VerfasserIn]
• Verfasst von: Gorzelanny, Christian [VerfasserIn]
• Titel: Chitosan functionalized poly-ε-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications
• Verf.angabe: Myriam G. Tardajos, Giuseppe Cama, Mamoni Dash, Lara Misseeuw, Tom Gheysens, Christian Gorzelanny, Tom Coenye, Peter Dubruel
• E-Jahr: 2018
• Jahr: 21 February 2018
• Umfang: 9 S.
• Fussnoten: Gesehen am 09.05.2019
• Titel Quelle: Enthalten in: Carbohydrate polymers
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1981
• Jahr Quelle: 2018
• Band/Heft Quelle: 191(2018), Seite 127-135
• ISSN Quelle: 1879-1344
• DOI: doi:10.1016/j.carbpol.2018.02.060
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Antibacterial
• Sach-SW: Chitosan
• Sach-SW: Poly-ε-caprolactone
• Sach-SW: Scaffolds
• Sach-SW: Surface functionalization
• K10plus-PPN: 1665026456

Abstract

Tissue engineering (TE) approaches often employ polymer-based scaffolds to provide support with a view to the improved regeneration of damaged tissues. The aim of this research was to develop a surface modification method for introducing chitosan as an antibacterial agent in both electrospun membranes and 3D printed poly-ε-caprolactone (PCL) scaffolds. The scaffolds were functionalized by grafting methacrylic acid N-hydroxysuccinimide ester (NHSMA) onto the surface after Ar-plasma/air activation. Subsequently, the newly-introduced NHS groups were used to couple with chitosan of various molecular weights (Mw). High Mw chitosan exhibited a better coverage of the surface as indicated by the higher N% detected by X-ray photoelectron spectroscopy (XPS) and the observations with either scanning electron microscopy (SEM)(for fibers) or Coomassie blue staining (for 3D-printed scaffolds). A lactate dehydrogenase assay (LDH) using L929 fibroblasts demonstrated the cell-adhesion and cell-viability capacity of the modified samples. The antibacterial properties against S. aureus ATCC 6538 and S. epidermidis ET13 revealed a slower bacterial growth rate on the surface of the chitosan modified scaffolds, regardless the chitosan Mw.