Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering

• Verfasst von: Goldberg-Bockhorn, Eva [VerfasserIn]
• Verfasst von: Rotter, Nicole [VerfasserIn]
• Verfasst von: Elsässer, Alexaner [VerfasserIn]
• Titel: Laser surface modification of decellularized extracellular cartilage matrix for cartilage tissue engineering
• Verf.angabe: Eva Goldberg-Bockhorn, Silke Schwarz, Rachana Subedi, Alexander Elsässer, Ricarda Riepl, Paul Walther, Ludwig Körber, Roman Breiter, Karl Stock, Nicole Rotter
• Jahr: 2018
• Jahr des Originals: 2017
• Umfang: 10 S.
• Fussnoten: First Online: 06 December 2017 ; Gesehen am 09.05.2019
• Titel Quelle: Enthalten in: Lasers in medical science
• Ort Quelle: London : Springer, 1986
• Jahr Quelle: 2018
• Band/Heft Quelle: 33(2018), 2, Seite 375-384
• ISSN Quelle: 1435-604X
• DOI: doi:10.1007/s10103-017-2402-8
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Carbon dioxide laser
• Sach-SW: Cartilage
• Sach-SW: Chondrocytes
• Sach-SW: Er:YAG laser
• Sach-SW: Surface modification
• Sach-SW: Tissue engineering
• K10plus-PPN: 1665056118

Abstract

The implantation of autologous cartilage as the gold standard operative procedure for the reconstruction of cartilage defects in the head and neck region unfortunately implicates a variety of negative effects at the donor site. Tissue-engineered cartilage appears to be a promising alternative. However, due to the complex requirements, the optimal material is yet to be determined. As demonstrated previously, decellularized porcine cartilage (DECM) might be a good option to engineer vital cartilage. As the dense structure of DECM limits cellular infiltration, we investigated surface modifications of the scaffolds by carbon dioxide (CO2) and Er:YAG laser application to facilitate the migration of chondrocytes inside the scaffold. After laser treatment, the scaffolds were seeded with human nasal septal chondrocytes and analyzed with respect to cell migration and formation of new extracellular matrix proteins. Histology, immunohistochemistry, SEM, and TEM examination revealed an increase of the scaffolds’ surface area with proliferation of cell numbers on the scaffolds for both laser types. The lack of cytotoxic effects was demonstrated by standard cytotoxicity testing. However, a thermal denaturation area seemed to hinder the migration of the chondrocytes inside the scaffolds, even more so after CO2 laser treatment. Therefore, the Er:YAG laser seemed to be better suitable. Further modifications of the laser adjustments or the use of alternative laser systems might be advantageous for surface enlargement and to facilitate migration of chondrocytes into the scaffold in one step.