Cartilage regeneration using decellularized cartilage matrix

• Verfasst von: Bomhard, Achim von [VerfasserIn]
• Verfasst von: Rotter, Nicole [VerfasserIn]
• Titel: Cartilage regeneration using decellularized cartilage matrix
• Titelzusatz: Long-term comparison of subcutaneous and intranasal placement in a rabbit model
• Verf.angabe: Achim von Bomhard, Alexander Elsaesser, Ricarda Riepl, Katharina Pippich, Joseph Faust, Silke Schwarz, Ludwig Koerber, Roman Breiter, Nicole Rotter
• E-Jahr: 2019
• Jahr: [April 2019]
• Umfang: 13 S.
• Illustrationen: Illustrationen
• Fussnoten: Available online 25 January 2019 ; Gesehen am 13.05.2019
• Titel Quelle: Enthalten in: Journal of cranio-maxillofacial surgery
• Ort Quelle: Oxford [u.a.] : Elsevier, 1987
• Jahr Quelle: 2019
• Band/Heft Quelle: 47(2019), 4, Seite 682-694
• ISSN Quelle: 1878-4119
• DOI: doi:10.1016/j.jcms.2019.01.010
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Biocompatibility
• Sach-SW: Cartilage reconstruction
• Sach-SW: Collagen implant
• Sach-SW: Decellularization
• Sach-SW: Long-term
• Sach-SW: Tissue engineering
• K10plus-PPN: 1665314036

Abstract

Autologous cartilage as donor tissue for various surgical reconstructions such as nasal septum regeneration is limited and associated with donor site morbidity. Our goal was to evaluate a new resorbable chondroconductive biomaterial made of decellularized porcine nasal septum cartilage compared with autologous native auricular cartilage as the gold standard. In order to examine the material and determine its long-term outcome further, we used subcutaneous implantation and septal implantation in an orthotopic rabbit model. In addition to non-seeded decellularized xenogenic cartilage, chondrocyte-seeded decellularized xenogenic cartilage was implanted as a septal replacement. After a three- or six-month period, the formation of newly synthesized cartilage extracellular matrix was evaluated immunohistochemically, whereas septal integrity and biocompatibility were evaluated histologically. The formation of the implanted neoseptum and form stability was analyzed by using 7-Tesla Magnetic Resonance Imaging. Good biocompatibility with no excessive rejection was demonstrated in all groups. Long-term stable and reliable septal reconstruction could be achieved in the study groups with or without cell seeding with autologous auricular chondrocytes. Autologous cell seeding was advantageous only with regard to septal perforations. Thus, cell seeding provides a benefit regarding long-term stability. However, because of slightly better biocompatibility, less pronounced septum deviation and the markedly lower effort involved, the non-seeded scaffold is favoured for possible clinical application.