Status: Bibliographieeintrag
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| Verfasst von: | Gunzelmann, Judith [VerfasserIn]  |
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| | Rüthnick, Diana [VerfasserIn] |
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| | Lin, Dennis Tien-chen [VerfasserIn] |
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| | Zhang, Wanlu [VerfasserIn] |
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| | Neuner, Annett [VerfasserIn] |
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| | Jäkle, Ursula [VerfasserIn] |
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| | Schiebel, Elmar [VerfasserIn] |
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| Titel: | The microtubule polymerase Stu2 promotes oligomerization of the γ-TuSC for cytoplasmic microtubule nucleation |
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| Verf.angabe: | Judith Gunzelmann, Diana Rüthnick, Tien-chen Lin, Wanlu Zhang, Annett Neuner, Ursula Jäkle, Elmar Schiebel |
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| E-Jahr: | 2018 |
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| Jahr: | September 26, 2018 |
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| Fussnoten: | Gesehen am 16.05.2019 |
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| Titel Quelle: | Enthalten in: eLife |
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| Ort Quelle: | Cambridge : eLife Sciences Publications, 2012 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 7(2018) Artikel-Nummer e39932 |
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| ISSN Quelle: | 2050-084X |
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| Abstract: | Stu2/XMAP215/ZYG-9/Dis1/Alp14/Msps/ch-TOG family members in association with with γ-tubulin complexes nucleate microtubules, but we know little about the interplay of these nucleation factors. Here, we show that the budding yeast Stu2 in complex with the γ-tubulin receptor Spc72 nucleates microtubules in vitro without the small γ-tubulin complex (γ-TuSC). Upon γ-TuSC addition, Stu2 facilitates Spc72-γ-TuSC interaction by binding to Spc72 and γ-TuSC. Stu2 together with Spc72-γ-TuSC increases microtubule nucleation in a process that is dependent on the TOG domains of Stu2. Importantly, these activities are also important for microtubule nucleation in vivo. Stu2 stabilizes Spc72-γ-TuSC at the minus end of cytoplasmic microtubules (cMTs) and an in vivo assay indicates that cMT nucleation requires the TOG domains of Stu2. Upon γ-tubulin depletion, we observed efficient cMT nucleation away from the spindle pole body (SPB), which was dependent on Stu2. Thus, γ-TuSC restricts cMT assembly to the SPB whereas Stu2 nucleates cMTs together with γ-TuSC and stabilizes γ-TuSC at the cMT minus end. |
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| DOI: | doi:10.7554/eLife.39932 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.7554/eLife.39932 |
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| | Volltext: https://doi.org/10.7554/eLife.39932 |
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| | DOI: https://doi.org/10.7554/eLife.39932 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | microtubule nucleation |
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| | Stu2 |
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| | TOG domain protein |
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| | γ-TuSC |
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| K10plus-PPN: | 1665811439 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ microtubule polymerase Stu2 promotes oligomerization of the γ-TuSC for cytoplasmic microtubule nucleation / Gunzelmann, Judith [VerfasserIn]; September 26, 2018 (Online-Ressource)
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