Green light for quantitative live-cell imaging in plants

• Verfasst von: Großmann, Guido [VerfasserIn]
• Verfasst von: Krebs, Melanie [VerfasserIn]
• Verfasst von: Maizel, Alexis [VerfasserIn]
• Titel: Green light for quantitative live-cell imaging in plants
• Verf.angabe: Guido Grossmann, Melanie Krebs, Alexis Maizel, Yvonne Stahl, Joop E.M. Vermeer and Thomas Ott
• E-Jahr: 2018
• Jahr: January 29, 2018
• Umfang: 14 S.
• Fussnoten: Gesehen am 17.05.2019
• Titel Quelle: Enthalten in: Journal of cell science
• Ort Quelle: Cambridge : Company of Biologists Limited, 1853
• Jahr Quelle: 2018
• Band/Heft Quelle: 131(2018,2) Artikel-Nummer jcs209270, 14 Seiten
• ISSN Quelle: 1477-9137
• DOI: doi:10.1242/jcs.209270
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1665881488

Abstract

Plants exhibit an intriguing morphological and physiological plasticity that enables them to thrive in a wide range of environments. To understand the cell biological basis of this unparalleled competence, a number of methodologies have been adapted or developed over the last decades that allow minimal or non-invasive live-cell imaging in the context of tissues. Combined with the ease to generate transgenic reporter lines in specific genetic backgrounds or accessions, we are witnessing a blooming in plant cell biology. However, the imaging of plant cells entails a number of specific challenges, such as high levels of autofluorescence, light scattering that is caused by cell walls and their sensitivity to environmental conditions. Quantitative live-cell imaging in plants therefore requires adapting or developing imaging techniques, as well as mounting and incubation systems, such as micro-fluidics. Here, we discuss some of these obstacles, and review a number of selected state-of-the-art techniques, such as two-photon imaging, light sheet microscopy and variable angle epifluorescence microscopy that allow high performance and minimal invasive live-cell imaging in plants.