Expression of inflammation-related intercellular adhesion molecules in cardiomyocytes in vitro and modulation by pro-inflammatory agents

• Verfasst von: El-Battrawy, Ibrahim [VerfasserIn]
• Verfasst von: Tueluemen, Erol [VerfasserIn]
• Verfasst von: Lang, Siegfried [VerfasserIn]
• Verfasst von: Akın, Ibrahim [VerfasserIn]
• Verfasst von: Behnes, Michael [VerfasserIn]
• Verfasst von: Zhou, Xiao-Bo [VerfasserIn]
• Verfasst von: Bugert, Peter [VerfasserIn]
• Verfasst von: Bieback, Karen [VerfasserIn]
• Verfasst von: Borggrefe, Martin [VerfasserIn]
• Verfasst von: Elmas, Elif [VerfasserIn]
• Titel: Expression of inflammation-related intercellular adhesion molecules in cardiomyocytes in vitro and modulation by pro-inflammatory agents
• Verf.angabe: Ibrahim El-Battrawy, Erol Tülümen, Siegfried Lang, Ibrahim Akin, Michael Behnes, Xiabo Zhou, Martin Mavany, Peter Bugert, Karen Bieback, Martin Borggrefe and Elif Elmas
• E-Jahr: 2016
• Jahr: May-June 2016
• Umfang: 5 S.
• Fussnoten: Gesehen am 21.05.2019
• Titel Quelle: Enthalten in: In vivo
• Ort Quelle: Kapandriti, Attiki : IIAR, 2004
• Jahr Quelle: 2016
• Band/Heft Quelle: 30(2016), 3, Seite 213-217
• ISSN Quelle: 1791-7549
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: cardiomyocytes in vitro model
• Sach-SW: CD31
• Sach-SW: cell adhesion molecules
• Sach-SW: inflammation
• K10plus-PPN: 1666042099

Abstract

Background: Cell-surface adhesion molecules regulate multiple intercellular and intracellular processes and play important roles in inflammation by facilitating leukocyte endothelial transmigration. Whether cardiomyocytes express surface-adhesion molecules related to inflammation and the effect of pro-inflammatory mediators remain unknown. Materials and Methods: In the present study, the expression of different cell-adhesion molecules (CD11a, CD11b, CD31, CD62P, CD162, F11 receptor and mucosal vascular addressin cell adhesion molecule 1 (MADCAM1)) and the effect of pro-inflammatory mediators were investigated in an in vitro model of human cardiomyocytes. Cells were supplied as a primary culture of cardiac alpha actin-positive cells from human heart tissue. The cells were incubated for 24 h with 1 U/ml thrombin or 700 ng/ml lipopolysaccharide (LPS) or with a combination of both. The expression of the cell adhesion molecules was measured by flow cytometry. Results. In cultured human cardiomyocytes, 22.8% of cells expressed CD31, 7.1% MADCAM1 and 2.6% F11R. CD11a, CD11b, CD62P and CD162 were expressed by fewer than 2% of the cells at baseline. CD31 expression increased on incubation of cardiomyocytes with thrombin by 26% (p<0.05) and with LPS by 26% (p=0.06). The combination of thrombin and LPS did not result in increased levels of CD31 (p>0.10). The pro-inflammatory agents LPS and thrombin had no effect on the expression of MADCAM1 and F11R. Conclusion: Inflammation-related cell-adhesion molecules CD31, MADCAM1 and F11R were shown to be expressed on the surface of human cardiomyocytes in an in vitro model. Incubation with LPS or thrombin resulted in increased expression of CD31, however, it did not modify the expression of the cell adhesion molecules MADCAM1 and F11R.