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Status: Bibliographieeintrag

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Verfasst von:Brenner, Nicole [VerfasserIn]   i
 Tabatabai, Julia [VerfasserIn]   i
 Schnitzler, Paul [VerfasserIn]   i
Titel:Validation of monoplex assays detecting antibodies against Corynebacterium diphtheriae and Clostridium tetani toxins, rubella virus and parvovirus B19 for incorporation into Multiplex Serology
Verf.angabe:Nicole Brenner, Julia Butt, Izaura Lima Bomfim, Julia Tabatabai, Michael Pawlita, Paul Schnitzler, Tim Waterboer
E-Jahr:2019
Jahr:28 January 2019
Umfang:10 S.
Fussnoten:Gesehen am 22.05.2019
Titel Quelle:Enthalten in: Methods
Ort Quelle:Orlando, Fla. : Academic Press, 1990
Jahr Quelle:2019
Band/Heft Quelle:158(2019), Seite 44-53
ISSN Quelle:1095-9130
Abstract:Serological assays detecting antibodies in serum or plasma samples are useful and versatile instruments to investigate an individual’s infection and vaccination history, e.g. for clinical diagnosis, personal risk evaluation, and seroepidemiological studies. Multiplex Serology is a suspension bead array-based high-throughput methodology for simultaneous measurement of antibodies against multiple pathogens in a single reaction vessel, thus economizing sample volume, measurement time, and costs. We developed and validated bead-based pathogen-specific Monoplex Serology assays, i.e. assays including only antigens for the respective pathogen, to detect antibodies against Corynebacterium diphtheriae and Clostridium tetani toxins, rubella virus and parvovirus B19. The developed assays expand the portfolio of existing pathogen-specific bead-based serology assays and can be efficiently incorporated into larger Multiplex Serology panels. The newly developed Monoplex Serology assays consist of only one antigen per infectious agent, expressed as Glutathione S-transferase-fusion proteins in E. coli. Specificity, sensitivity and Cohen’s kappa statistics in comparison with routine clinical diagnostic assays were calculated for serum dilutions 1:100 and 1:1000. All pathogen-specific assays were successfully validated at both serum dilutions with the exception of rubella Monoplex Serology which showed impaired sensitivity (57.6%) at dilution 1:1000. Specificities of successfully validated Monoplex Serology assays ranged from 85.6% to 100.0% (median: 91.7%), and sensitivities from 81.3% to 95.8% (median: 90.9%); agreement with the reference assays ranged from substantial to almost perfect (kappa: 0.66–0.86, median: 0.78). Statistical performance and slim assay design enable efficient incorporation of the developed assays into Multiplex Serology.
DOI:doi:10.1016/j.ymeth.2019.01.013
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.ymeth.2019.01.013
 Volltext: http://www.sciencedirect.com/science/article/pii/S1046202318301993
 DOI: https://doi.org/10.1016/j.ymeth.2019.01.013
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:High-throughput assay
 Multiplex
 Parvovirus B19
 Serology
 Vaccine-preventable diseases
K10plus-PPN:1666088307
Verknüpfungen:→ Zeitschrift

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