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Status: Bibliographieeintrag

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Verfasst von:Grünvogel, Oliver [VerfasserIn]   i
 Colasanti, Ombretta [VerfasserIn]   i
 Lee, Ji Young [VerfasserIn]   i
 Klöss, Volker [VerfasserIn]   i
 Esser-Nobis, Katharina [VerfasserIn]   i
 Mutz, Pascal [VerfasserIn]   i
 Hoffmann, Katrin [VerfasserIn]   i
 Mehrabi, Arianeb [VerfasserIn]   i
 Koschny, Ronald [VerfasserIn]   i
 Gotthardt, Daniel [VerfasserIn]   i
 Schnitzler, Paul [VerfasserIn]   i
 Binder, Marco [VerfasserIn]   i
 Bartenschlager, Ralf [VerfasserIn]   i
 Dalpke, Alexander [VerfasserIn]   i
 Lohmann, Volker [VerfasserIn]   i
Titel:Secretion of Hepatitis C Virus replication intermediates reduces activation of toll-like receptor 3 in hepatocytes
Verf.angabe:Oliver Grünvogel, Ombretta Colasanti, Ji-Young Lee, Volker Klöss, Sandrine Belouzard, Anna Reustle, Katharina Esser-Nobis, Jasper Hesebeck-Brinckmann, Pascal Mutz, Katrin Hoffmann, Arianeb Mehrabi, Ronald Koschny, Florian W.R. Vondran, Daniel Gotthardt, Paul Schnitzler, Christoph Neumann-Haefelin, Robert Thimme, Marco Binder, Ralf Bartenschlager, Jean Dubuisson, Alexander H. Dalpke, and Volker Lohmann
E-Jahr:2018
Jahr:11 March 2018
Umfang:31 S.
Fussnoten:Gesehen am 22.05.2019
Titel Quelle:Enthalten in: Gastroenterology
Ort Quelle:Stanford, Calif. : HighWire Press, 1965
Jahr Quelle:2018
Band/Heft Quelle:154(2018), 8, Seite 2237-2251.e16
ISSN Quelle:1528-0012
Abstract:Background & Aims - Hepatitis C virus (HCV) infections most often result in chronic outcomes, although the virus constantly produces replication intermediates, in particular double-stranded RNA (dsRNA), representing potent inducers of innate immunity. We aimed to characterize the fate of HCV dsRNA in hepatocyte cultures to identify mechanisms contributing to viral persistence in presence of an active innate immune response. - Methods - We analyzed hepatocyte-based culture models for HCV for induction of innate immunity, secretion of virus positive- or negative-strand RNA, and viral replication using different quantification methods and microscopy techniques. Expression of pattern recognition receptors was reconstituted in hepatoma cells by lentiviral transduction. - Results - HCV-infected cells secrete substantial amounts of virus positive- and negative-strand RNAs in extracellular vesicles (EVs), toward the apical and basolateral domain of hepatocytes. Secretion of negative-strand RNA was independent from virus production, and viral RNA secreted in EVs contained higher relative amounts of negative-strands, indicating that mostly virus dsRNA is released. A substantial part of viral replication complexes and dsRNA was found in the endosomal compartment and multivesicular bodies, indicating that secretion of HCV replication intermediates is mediated by the exosomal pathway. Block of vesicle release in HCV-positive cells increased intracellular dsRNA levels and increased activation of toll-like receptor 3, inhibiting HCV replication. - Conclusions - Using hepatocyte-based culture models for HCV, we found a portion of HCV dsRNA intermediates to be released from infected cells in EVs, which reduces activation of toll-like receptor 3. This represents a novel mechanism how HCV evades host immune responses, potentially contributing to viral persistence.
DOI:doi:10.1053/j.gastro.2018.03.020
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1053/j.gastro.2018.03.020
 Volltext: http://www.sciencedirect.com/science/article/pii/S0016508518303093
 DOI: https://doi.org/10.1053/j.gastro.2018.03.020
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Escape
 Exosomes
 Rab27a
 Virology
K10plus-PPN:1666098752
Verknüpfungen:→ Zeitschrift

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