Combining adhesive nanostructured surfaces and costimulatory signals to increase T Cell activation

• Verfasst von: Guasch, Judith [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Combining adhesive nanostructured surfaces and costimulatory signals to increase T Cell activation
• Verf.angabe: Judith Guasch, Marco Hoffmann, Jennifer Diemer, Hossein Riahinezhad, Stefanie Neubauer, Horst Kessler, and Joachim P. Spatz
• E-Jahr: 2018
• Jahr: 8 August 2018
• Umfang: 6 S.
• Fussnoten: Gesehen am 23.05.2019
• Titel Quelle: Enthalten in: Nano letters
• Ort Quelle: Washington, DC : ACS Publ., 2001
• Jahr Quelle: 2018
• Band/Heft Quelle: 18(2018), 9, Seite 5899-5904
• ISSN Quelle: 1530-6992
• DOI: doi:10.1021/acs.nanolett.8b02588
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1666187240

Abstract

Adoptive cell therapies are showing very promising results in the fight against cancer. However, these therapies are expensive and technically challenging in part due to the need of a large number of specific T cells, which must be activated and expanded in vitro. Here we describe a method to activate primary human T cells using a combination of nanostructured surfaces functionalized with the stimulating anti-CD3 antibody and the peptidic sequence arginine-glycine-aspartic acid, as well as costimulatory agents (anti-CD28 antibody and a cocktail of phorbol 12-myristate 13-acetate, ionomycin, and protein transport inhibitors). Thus, we propose a method that combines nanotechnology with cell biology procedures to efficiently produce T cells in the laboratory, challenging the current state-of-the-art expansion methodologies.