Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Schäfer, Michael [VerfasserIn]  |
| Gross, Wolfgang [VerfasserIn]  |
| Gebhard, Martha-Maria [VerfasserIn]  |
Titel: | Hearts during ischemia with or without HTK-protection analysed by dielectric spectroscopy |
Verf.angabe: | Michael Schaefer, Wolfgang Gross and Martha-Maria Gebhard |
E-Jahr: | 2018 |
Jahr: | 26 February 2018 |
Teil: | volume:39 |
| year:2018 |
| number:2 |
Fussnoten: | Gesehen am 24.05.2019 |
Titel Quelle: | Enthalten in: Physiological measurement |
Ort Quelle: | Bristol : IOP Publ., 1980 |
Jahr Quelle: | 2018 |
Band/Heft Quelle: | 39(2018,2) Artikel-Nummer 025002, ? Seiten |
ISSN Quelle: | 1361-6579 |
Abstract: | Objective: We investigated canine hearts during ischemia after aortic cross clamping (UI, n = 20) and after HTK-cardioplegia (HTK, n = 24) at 35 °C, 25 °C, 15 °C, and 5 °C with the aim to compare tissue changes caused by the activity of anaerobic metabolism(AAM), cell membrane destruction(CD), and gap junction uncoupling(GJU). Approach: We measured continuously the complex dielectric spectrum(DS), ATP- and lactate content, extracellular pH, and rigor contracture. To identify changes in DS caused by AAM, CD, and GJU we performed additional experiments on the gap junction-free skeletal muscle. We used heart model simulations to calculate the effect of temperature. Main results: AAM affected the DS at 10 MHz and we found a strong correlation between DS and the proton concentration with a maximum of DS at 10 mmol g−1 dry weight in ATP-concentration. The time of GJU was detected by a characteristic increase in DS and CD by a characteristic decrease at 13 kHz. In comparison to UI, GJU, AAM and CD were delayed by HTK and by hypothermia, indicating a minimization of energy consumption and an improved preservation of tissue structure. Significance: The novel findings were that in UI at 5 °C GJU occurred earlier and AAM remained constant, indicating a less effective preservation in UI by deep hypothermia in contrast to HTK. |
DOI: | doi:10.1088/1361-6579/aaa575 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1088/1361-6579/aaa575 |
| Volltext: https://doi.org/10.1088%2F1361-6579%2Faaa575 |
| DOI: https://doi.org/10.1088/1361-6579/aaa575 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1666283436 |
Verknüpfungen: | → Zeitschrift |
Hearts during ischemia with or without HTK-protection analysed by dielectric spectroscopy / Schäfer, Michael [VerfasserIn]; 26 February 2018 (Online-Ressource)
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