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Status: Bibliographieeintrag

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Verfasst von:Brenner, Nicole [VerfasserIn]   i
 Schnitzler, Paul [VerfasserIn]   i
 Pawlita, Michael [VerfasserIn]   i
Titel:Validation of multiplex serology for human hepatitis viruses B and C, human T-lymphotropic virus 1 and Toxoplasma gondii
Verf.angabe:Nicole Brenner, Alexander J. Mentzer, Julia Butt, Kathrin L. Braband, Angelika Michel, Katie Jeffery, Paul Klenerman, Barbara Gärtner, Paul Schnitzler, Adrian Hill, Graham Taylor, Maria A. Demontis, Edward Guy, Stephen J. Hadfield, Rachael Almond, Naomi Allen, Michael Pawlita, and Tim Waterboer
E-Jahr:2019
Jahr:January 7, 2019
Umfang:21 S.
Teil:volume:14
 year:2019
 number:1
 extent:21
Fussnoten:Gesehen am 29.05.2019
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2019
Band/Heft Quelle:14(2019,1) Artikel-Nummere0210407, 21 Seiten
ISSN Quelle:1932-6203
Abstract:Multiplex Serology is a high-throughput technology developed to simultaneously measure specific serum antibodies against multiple pathogens in one reaction vessel. Serological assays for hepatitis B (HBV) and C (HCV) viruses, human T-lymphotropic virus 1 (HTLV-1) and the protozoan parasite Toxoplasma gondii (T. gondii) were developed and validated against established reference assays. For each pathogen, between 3 and 5 specific antigens were recombinantly expressed as GST-tag fusion proteins in Escherichia coli and tested in Monoplex Serology, i.e. assays restricted to the antigens from one particular pathogen. For each of the four pathogen-specific Monoplex assays, overall seropositivity was defined using two pathogen-specific antigens. In the case of HBV Monoplex Serology, the detection of past natural HBV infection was validated based on two independent reference panels resulting in sensitivities of 92.3% and 93.0%, and specificities of 100% in both panels. Validation of HCV and HTLV-1 Monoplex Serology resulted in sensitivities of 98.0% and 95.0%, and specificities of 96.2% and 100.0%, respectively. The Monoplex Serology assay for T. gondii was validated with a sensitivity of 91.2% and specificity of 92.0%. The developed Monoplex Serology assays largely retained their characteristics when they were included in a multiplex panel (i.e. Multiplex Serology), containing additional antigens from a broad range of other pathogens. Thus HBV, HCV, HTLV-1 and T. gondii Monoplex Serology assays can efficiently be incorporated into Multiplex Serology panels tailored for application in seroepidemiological studies.
DOI:doi:10.1371/journal.pone.0210407
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0210407
 Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0210407
 DOI: https://doi.org/10.1371/journal.pone.0210407
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Antibodies
 Antigens
 Enzyme-linked immunoassays
 Hepatitis C virus
 HTLV-1
 Protozoan infections
 Serology
 Toxoplasma gondii
K10plus-PPN:1666501654
Verknüpfungen:→ Zeitschrift

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