Online-Ressource | |
Verfasst von: | Kirschner, Stefanie [VerfasserIn] |
Mürle, Bettina [VerfasserIn] | |
Felix, Manuela [VerfasserIn] | |
Arns, Anna Maria [VerfasserIn] | |
Groden, Christoph [VerfasserIn] | |
Wenz, Frederik [VerfasserIn] | |
Hug, Andreas [VerfasserIn] | |
Glatting, Gerhard [VerfasserIn] | |
Giordano, Frank Anton [VerfasserIn] | |
Titel: | Imaging of orthotopic glioblastoma xenografts in mice using a clinical CT scanner |
Titelzusatz: | comparison with micro-CT and histology |
Verf.angabe: | Stefanie Kirschner, Bettina Mürle, Manuela Felix, Anna Arns, Christoph Groden, Frederik Wenz, Andreas Hug, Gerhard Glatting, Martin Kramer, Frank A. Giordano, Marc A. Brockmann |
E-Jahr: | 2016 |
Jahr: | November 9, 2016 |
Umfang: | 13 S. |
Fussnoten: | Gesehen am 29.05.2019 |
Titel Quelle: | Enthalten in: PLOS ONE |
Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
Jahr Quelle: | 2016 |
Band/Heft Quelle: | 11(2016,11) Artikel-Nummer e0165994, 13 Seiten |
ISSN Quelle: | 1932-6203 |
Abstract: | Purpose There is an increasing need for small animal in vivo imaging in murine orthotopic glioma models. Because dedicated small animal scanners are not available ubiquitously, the applicability of a clinical CT scanner for visualization and measurement of intracerebrally growing glioma xenografts in living mice was validated. Materials and Methods 2.5x106 U87MG cells were orthotopically implanted in NOD/SCID/ᵞc-/- mice (n = 9). Mice underwent contrast-enhanced (300 μl Iomeprol i.v.) imaging using a micro-CT (80 kV, 75 μAs, 360° rotation, 1,000 projections, scan time 33 s, resolution 40 x 40 x 53 μm) and a clinical CT scanner (4-row multislice detector; 120 kV, 150 mAs, slice thickness 0.5 mm, feed rotation 0.5 mm, resolution 98 x 98 x 500 μm). Mice were sacrificed and the brain was worked up histologically. In all modalities tumor volume was measured by two independent readers. Contrast-to-noise ratio (CNR) and Signal-to-noise ratio (SNR) were measured from reconstructed CT-scans (0.5 mm slice thickness; n = 18). Results Tumor volumes (mean±SD mm3) were similar between both CT-modalities (micro-CT: 19.8±19.0, clinical CT: 19.8±18.8; Wilcoxon signed-rank test p = 0.813). Moreover, between reader analyses for each modality showed excellent agreement as demonstrated by correlation analysis (Spearman-Rho >0.9; p<0.01 for all correlations). Histologically measured tumor volumes (11.0±11.2) were significantly smaller due to shrinkage artifacts (p<0.05). CNR and SNR were 2.1±1.0 and 1.1±0.04 for micro-CT and 23.1±24.0 and 1.9±0.7 for the clinical CTscanner, respectively. Conclusion Clinical CT scanners may reliably be used for in vivo imaging and volumetric analysis of brain tumor growth in mice. |
DOI: | doi:10.1371/journal.pone.0165994 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt. Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0165994 |
Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0165994 | |
DOI: https://doi.org/10.1371/journal.pone.0165994 | |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Cancer treatment |
Computed axial tomography | |
Glioma | |
Histology | |
In vivo imaging | |
Magnetic resonance imaging | |
Neuroimaging | |
Small animals | |
K10plus-PPN: | 1666523674 |
Verknüpfungen: | → Zeitschrift |