| Online-Ressource |
Verfasst von: | Westhauser, Fabian [VerfasserIn]  |
| Senger, Anne-Sophie [VerfasserIn]  |
| Obert, David Peter [VerfasserIn]  |
| Ciraldo, Francesca E. [VerfasserIn]  |
| Schuhladen, Katharina [VerfasserIn]  |
| Schmidmaier, Gerhard [VerfasserIn]  |
| Moghaddam-Alvandi, Arash [VerfasserIn]  |
| Boccaccini, Aldo R. [VerfasserIn]  |
Titel: | Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds |
Verf.angabe: | Fabian Westhauser, Anne-Sophie Senger, David Obert, Francesca E. Ciraldo, Katharina Schuhladen, Gerhard Schmidmaier, Arash Moghaddam, Aldo R. Boccaccini |
Jahr: | 2019 |
Jahr des Originals: | 2018 |
Umfang: | 12 S. |
Fussnoten: | First published: 07 December 2018 ; Gesehen am 29.05.2019 |
Titel Quelle: | Enthalten in: Journal of tissue engineering and regenerative medicine |
Ort Quelle: | Hoboken, NJ [u.a.] : Wiley, 2007 |
Jahr Quelle: | 2019 |
Band/Heft Quelle: | 13(2019), 2, Seite 179-190 |
ISSN Quelle: | 1932-7005 |
Abstract: | Recent studies have demonstrated that surface characteristics, porosity, and mechanical strength of three-dimensional 45S5-type bioactive glass (BG)-based scaffolds are directly correlated with osteogenic properties. Three-dimensional BG-based scaffolds obtained from maritime natural sponges (MNSs) as sacrificial templates exhibit the required morphological properties; however, no in vivo data about the osteogenic features are available. In this study, uncoated (Group A) and gelatin-coated (Group B) crystalline MNS-obtained BG-based scaffolds were evaluated mechanically and seeded with human mesenchymal stem cells prior to subcutaneous implantation in immunodeficient mice. Before implantation and after explantation, micro-computed tomography scans were conducted, and scaffolds were finally subjected to histomorphometry. Scaffolds of both groups showed bone formation. However, Group B scaffolds performed distinctly better as indicated by a significant increase in scaffold volume (8.95%, p = 0.039) over the implantation period compared with a nonsignificant increase of 5.26% in Group A scaffolds in micro-computed tomography analysis. Furthermore, percentage bone area was 10.33% (±1.18%) in the Group B scaffolds, which was significantly (p = 0.007) higher compared with the 8.53% (±0.77%) in the Group A scaffolds in histomorphometry. Compressive strength was enhanced significantly by gelatin coating (9 ± 2 vs. 4 ± 1 MPa; p = 0.029). The presence of gelatin on the remnant parts was verified by scanning electron microscopy and X-ray spectroscopy, demonstrating the coatings' resilience. MNS-obtained BG-based scaffolds were thus confirmed to exhibit osteogenic properties in vivo that can significantly be enhanced by gelatin coating. |
DOI: | doi:10.1002/term.2780 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/term.2780 |
| Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/term.2780 |
| DOI: https://doi.org/10.1002/term.2780 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | bioactive glass |
| bone formation |
| gelatin coating |
| human mesenchymal stem cells |
| in vivo |
| maritime sponge |
| micro-computed tomography |
| tissue engineering |
K10plus-PPN: | 1666536016 |
Verknüpfungen: | → Zeitschrift |
Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds / Westhauser, Fabian [VerfasserIn]; 2019 (Online-Ressource)