Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds

• Verfasst von: Westhauser, Fabian [VerfasserIn]
• Verfasst von: Senger, Anne-Sophie [VerfasserIn]
• Verfasst von: Obert, David Peter [VerfasserIn]
• Verfasst von: Ciraldo, Francesca E. [VerfasserIn]
• Verfasst von: Schuhladen, Katharina [VerfasserIn]
• Verfasst von: Schmidmaier, Gerhard [VerfasserIn]
• Verfasst von: Moghaddam-Alvandi, Arash [VerfasserIn]
• Verfasst von: Boccaccini, Aldo R. [VerfasserIn]
• Titel: Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds
• Verf.angabe: Fabian Westhauser, Anne-Sophie Senger, David Obert, Francesca E. Ciraldo, Katharina Schuhladen, Gerhard Schmidmaier, Arash Moghaddam, Aldo R. Boccaccini
• Jahr: 2019
• Jahr des Originals: 2018
• Umfang: 12 S.
• Fussnoten: First published: 07 December 2018 ; Gesehen am 29.05.2019
• Titel Quelle: Enthalten in: Journal of tissue engineering and regenerative medicine
• Ort Quelle: Hoboken, NJ [u.a.] : Wiley, 2007
• Jahr Quelle: 2019
• Band/Heft Quelle: 13(2019), 2, Seite 179-190
• ISSN Quelle: 1932-7005
• DOI: doi:10.1002/term.2780
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: bioactive glass
• Sach-SW: bone formation
• Sach-SW: gelatin coating
• Sach-SW: human mesenchymal stem cells
• Sach-SW: in vivo
• Sach-SW: maritime sponge
• Sach-SW: micro-computed tomography
• Sach-SW: tissue engineering
• K10plus-PPN: 1666536016

Abstract

Recent studies have demonstrated that surface characteristics, porosity, and mechanical strength of three-dimensional 45S5-type bioactive glass (BG)-based scaffolds are directly correlated with osteogenic properties. Three-dimensional BG-based scaffolds obtained from maritime natural sponges (MNSs) as sacrificial templates exhibit the required morphological properties; however, no in vivo data about the osteogenic features are available. In this study, uncoated (Group A) and gelatin-coated (Group B) crystalline MNS-obtained BG-based scaffolds were evaluated mechanically and seeded with human mesenchymal stem cells prior to subcutaneous implantation in immunodeficient mice. Before implantation and after explantation, micro-computed tomography scans were conducted, and scaffolds were finally subjected to histomorphometry. Scaffolds of both groups showed bone formation. However, Group B scaffolds performed distinctly better as indicated by a significant increase in scaffold volume (8.95%, p = 0.039) over the implantation period compared with a nonsignificant increase of 5.26% in Group A scaffolds in micro-computed tomography analysis. Furthermore, percentage bone area was 10.33% (±1.18%) in the Group B scaffolds, which was significantly (p = 0.007) higher compared with the 8.53% (±0.77%) in the Group A scaffolds in histomorphometry. Compressive strength was enhanced significantly by gelatin coating (9 ± 2 vs. 4 ± 1 MPa; p = 0.029). The presence of gelatin on the remnant parts was verified by scanning electron microscopy and X-ray spectroscopy, demonstrating the coatings' resilience. MNS-obtained BG-based scaffolds were thus confirmed to exhibit osteogenic properties in vivo that can significantly be enhanced by gelatin coating.