Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Akanji, Oluwadamilola [VerfasserIn]   i
 Weinzierl, Nina [VerfasserIn]   i
 Schubert, Rudolf [VerfasserIn]   i
 Schilling, Lothar [VerfasserIn]   i
Titel:Acid sensing ion channels in rat cerebral arteries
Titelzusatz:probing the expression pattern and vasomotor activity
Verf.angabe:Oluwadamilola Akanji, Nina Weinzierl, Rudolf Schubert, Lothar Schilling
E-Jahr:2019
Jahr:23 April 2019
Umfang:8 S.
Fussnoten:Gesehen am 03.06.2019
Titel Quelle:Enthalten in: Life sciences
Ort Quelle:New York, NY [u.a.] : Elsevier Science, 1963
Jahr Quelle:2019
Band/Heft Quelle:227(2019), Seite 193-200
ISSN Quelle:1879-0631
Abstract:Aims - The recent identification of acid sensing ion channels (ASICs) in vascular beds suggests their possible involvement in modulating vasomotor tone. Therefore, we investigated the gene expression profiles of ASIC subtypes in the middle cerebral artery (MCA) of Wistar rats and the functional implication of ASICs in acidosis-induced relaxation as well as maintenance of resting tension. - Main methods - Real time PCR was employed to study the pattern of ASIC mRNA expression in the MCA wall in comparison with (i) matching brain tissue samples and (ii) arteries cultured for 24h and 48h. The functional implication regarding vasomotor response to acidosis and maintenance of resting tension was assessed using in vitro myography. - Key findings - A robust mRNA expression of ASIC-1, -2 and -4 was found in brain tissue samples and to a lower extent in freshly isolated MCA. In the MCA wall, short term culture induced a down-regulation of ASIC-1 and -2 expression without any remarkable change in ASIC-4 expression. Acidosis induced a pH-related relaxation of freshly isolated MCA ring segments, being more pronounced after short term culture. Incubation with the ASIC blocker amiloride moderately enhanced acidosis-induced relaxation, in cultured MCAs somewhat stronger than in freshly isolated vessels. In addition, amiloride resulted in a decrease of resting tension, albeit only in freshly isolated MCA. - Significance - Our results comprehensively describe ASIC subtype composition in the rat MCA in physiological and pathological conditions and strongly suggest the involvement of ASICs in the modulation of vasomotor responses under conditions of normal or decreased pH values.
DOI:doi:10.1016/j.lfs.2019.04.054
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.lfs.2019.04.054
 Volltext: http://www.sciencedirect.com/science/article/pii/S0024320519303169
 DOI: https://doi.org/10.1016/j.lfs.2019.04.054
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Acid-sensing ion channels
 Cerebral artery
 Extracellular acidosis
 Short-term culture
 Vasorelaxation
K10plus-PPN:1666644757
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68394865   QR-Code
zum Seitenanfang