| |  Online-Ressource |
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| Verfasst von: | Walch-Rückheim, Barbara [VerfasserIn]  |
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| | Kiefer, Ruth Annabelle [VerfasserIn] |
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| | Geginat, Gernot [VerfasserIn] |
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| | Schmitt, Manfred J. [VerfasserIn] |
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| | Breinig, Frank [VerfasserIn] |
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| Titel: | Coexpression of human perforin improves yeast-mediated delivery of DNA and mRNA to mammalian antigen-presenting cells |
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| Verf.angabe: | B. Walch-Rückheim, R. Kiefer, G. Geginat, M.J. Schmitt and F. Breinig |
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| Jahr: | 2016 |
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| Jahr des Originals: | 2015 |
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| Umfang: | 5 S. |
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| Fussnoten: | Published online 20 August 2015 ; Gesehen am 13.06.2019 |
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| Titel Quelle: | Enthalten in: Gene therapy |
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| Ort Quelle: | London : Nature Publ. Group, 1997 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 23(2016), 1, Seite 103-107 |
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| ISSN Quelle: | 1476-5462 |
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| Abstract: | Previous studies underlined the capacity of recombinant yeast as efficient vehicle for the targeted delivery of functional nucleic acids as well as proteinaceous antigens to mammalian antigen-presenting cells (APCs). To improve this yeast-mediated cargo transport into APCs, we investigated the impact of coexpression of the human membrane-perturbing protein perforin in comparison with bacterial listeriolysin O (LLO) on the yeast-based delivery of DNA, mRNA and proteins to mammalian APCs. In contrast to LLO, a cholesterol-dependent pore-forming toxin of Listeria, intracellular expression of human perforin in Saccharomyces cerevisiae had no impact on yeast cell viability, while its coexpression significantly increased translocation of ovalbumin and subsequent activation of ovalbumin-specific T lymphocytes. Likewise, perforin improved the expression of the model antigen enhanced green fluorescent protein after yeast-mediated DNA and mRNA delivery, whereas LLO was only able to enhance DNA delivery. Taken together, our data show that human perforin, besides bacterial hemolysins, represents a promising means to improve the yeast-mediated delivery of functional nucleic acids and proteins to mammalian APCs. |
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| DOI: | doi:10.1038/gt.2015.77 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/gt.2015.77 |
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| | Volltext: https://www.nature.com/articles/gt201577 |
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| | Volltext: http://www.nature.com/gt/journal/v23/n1/abs/gt201577a.html |
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| | DOI: https://doi.org/10.1038/gt.2015.77 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 839818653 |
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| Verknüpfungen: | → Zeitschrift |
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Coexpression of human perforin improves yeast-mediated delivery of DNA and mRNA to mammalian antigen-presenting cells / Walch-Rückheim, Barbara [VerfasserIn]; 2016 (Online-Ressource)
