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Verfasst von:Bothe, Friederike [VerfasserIn]   i
 Knauf, Anne-Kathrin [VerfasserIn]   i
 Hesse, Eliane [VerfasserIn]   i
 Lotz, Benedict [VerfasserIn]   i
 Groll, Jürgen [VerfasserIn]   i
 Werner, Carsten [VerfasserIn]   i
 Richter, Wiltrud [VerfasserIn]   i
 Hagmann, Sébastien [VerfasserIn]   i
Titel:Treatment of focal cartilage defects in minipigs with zonal chondrocyte/mesenchymal progenitor cell constructs
Verf.angabe:Friederike Bothe, Anne-Kathrin Deubel, Eliane Hesse, Benedict Lotz, Jürgen Groll, Carsten Werner, Wiltrud Richter and Sebastien Hagmann
E-Jahr:2019
Jahr:2 February 2019
Umfang:17 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 18.06.2019
Titel Quelle:Enthalten in: International journal of molecular sciences
Ort Quelle:Basel : Molecular Diversity Preservation International, 2000
Jahr Quelle:2019
Band/Heft Quelle:20 (2019,3) Artikel-Nummer 653, Seite 1-17, 17 Seiten
ISSN Quelle:1422-0067
 1661-6596
Abstract:Despite advances in cartilage repair strategies, treatment of focal chondral lesions remains an important challenge to prevent osteoarthritis. Articular cartilage is organized into several layers and lack of zonal organization of current grafts is held responsible for insufficient biomechanical and biochemical quality of repair-tissue. The aim was to develop a zonal approach for cartilage regeneration to determine whether the outcome can be improved compared to a non-zonal strategy. Hydrogel-filled polycaprolactone (PCL)-constructs with a chondrocyte-seeded upper-layer deemed to induce hyaline cartilage and a mesenchymal stromal cell (MSC)-containing bottom-layer deemed to induce calcified cartilage were compared to chondrocyte-based non-zonal grafts in a minipig model. Grafts showed comparable hardness at implantation and did not cause visible signs of inflammation. After 6 months, X-ray microtomography (µCT)-analysis revealed significant bone-loss in both treatment groups compared to empty controls. PCL-enforcement and some hydrogel-remnants were retained in all defects, but most implants were pressed into the subchondral bone. Despite important heterogeneities, both treatments reached a significantly lower modified O’Driscoll-score compared to empty controls. Thus, PCL may have induced bone-erosion during joint loading and misplacement of grafts in vivo precluding adequate permanent orientation of zones compared to surrounding native cartilage.
DOI:doi:10.3390/ijms20030653
URL:Volltext ; Verlag: https://doi.org/10.3390/ijms20030653
 Volltext: https://www.mdpi.com/1422-0067/20/3/653
 DOI: https://doi.org/10.3390/ijms20030653
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:cartilage repair
 chondrocyte
 minipig
 MSC
 osteochondral defect
 starPEG hydrogel
 tissue engineering
 zonal construct
K10plus-PPN:1667567306
Verknüpfungen:→ Zeitschrift
 
 
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