Treatment of focal cartilage defects in minipigs with zonal chondrocyte/mesenchymal progenitor cell constructs

• Verfasst von: Bothe, Friederike [VerfasserIn]
• Verfasst von: Knauf, Anne-Kathrin [VerfasserIn]
• Verfasst von: Hesse, Eliane [VerfasserIn]
• Verfasst von: Lotz, Benedict [VerfasserIn]
• Verfasst von: Groll, Jürgen [VerfasserIn]
• Verfasst von: Werner, Carsten [VerfasserIn]
• Verfasst von: Richter, Wiltrud [VerfasserIn]
• Verfasst von: Hagmann, Sébastien [VerfasserIn]
• Titel: Treatment of focal cartilage defects in minipigs with zonal chondrocyte/mesenchymal progenitor cell constructs
• Verf.angabe: Friederike Bothe, Anne-Kathrin Deubel, Eliane Hesse, Benedict Lotz, Jürgen Groll, Carsten Werner, Wiltrud Richter and Sebastien Hagmann
• E-Jahr: 2019
• Jahr: 2 February 2019
• Umfang: 17 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 18.06.2019
• Titel Quelle: Enthalten in: International journal of molecular sciences
• Ort Quelle: Basel : Molecular Diversity Preservation International, 2000
• Jahr Quelle: 2019
• Band/Heft Quelle: 20 (2019,3) Artikel-Nummer 653, Seite 1-17, 17 Seiten
• ISSN Quelle: 1422-0067
• ISSN Quelle: 1661-6596
• DOI: doi:10.3390/ijms20030653
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: cartilage repair
• Sach-SW: chondrocyte
• Sach-SW: minipig
• Sach-SW: MSC
• Sach-SW: osteochondral defect
• Sach-SW: starPEG hydrogel
• Sach-SW: tissue engineering
• Sach-SW: zonal construct
• K10plus-PPN: 1667567306
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Despite advances in cartilage repair strategies, treatment of focal chondral lesions remains an important challenge to prevent osteoarthritis. Articular cartilage is organized into several layers and lack of zonal organization of current grafts is held responsible for insufficient biomechanical and biochemical quality of repair-tissue. The aim was to develop a zonal approach for cartilage regeneration to determine whether the outcome can be improved compared to a non-zonal strategy. Hydrogel-filled polycaprolactone (PCL)-constructs with a chondrocyte-seeded upper-layer deemed to induce hyaline cartilage and a mesenchymal stromal cell (MSC)-containing bottom-layer deemed to induce calcified cartilage were compared to chondrocyte-based non-zonal grafts in a minipig model. Grafts showed comparable hardness at implantation and did not cause visible signs of inflammation. After 6 months, X-ray microtomography (µCT)-analysis revealed significant bone-loss in both treatment groups compared to empty controls. PCL-enforcement and some hydrogel-remnants were retained in all defects, but most implants were pressed into the subchondral bone. Despite important heterogeneities, both treatments reached a significantly lower modified O’Driscoll-score compared to empty controls. Thus, PCL may have induced bone-erosion during joint loading and misplacement of grafts in vivo precluding adequate permanent orientation of zones compared to surrounding native cartilage.