RETRACTED ARTICLE: Pancreatic cancer-initiating cell exosome message transfer into noncancer-initiating cells

• Verfasst von: Wang, Zhe [VerfasserIn]
• Verfasst von: Sun, Hanxue [VerfasserIn]
• Verfasst von: Provaznik, Jan [VerfasserIn]
• Verfasst von: Hackert, Thilo [VerfasserIn]
• Verfasst von: Zöller, Margot [VerfasserIn]
• Titel: RETRACTED ARTICLE: Pancreatic cancer-initiating cell exosome message transfer into noncancer-initiating cells
• Titelzusatz: the importance of CD44v6 in reprogramming
• Verf.angabe: Zhe Wang, Hanxue Sun, Jan Provaznik, Thilo Hackert and Margot Zöller
• E-Jahr: 2019
• Jahr: 19 March 2019
• Umfang: 20 S.
• Fussnoten: Gesehen am 18.06.2019
• Titel Quelle: Enthalten in: Journal of experimental & clinical cancer research
• Ort Quelle: Berlin : Springer, 2008
• Jahr Quelle: 2019
• Band/Heft Quelle: 38(2019), Artikel-ID 132, Seite 1-20
• ISSN Quelle: 1756-9966
• DOI: doi:10.1186/s13046-019-1129-8
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Errata: Wang, Zhe, 1979 - : Correction: Pancreatic cancer-initiating cell exosome message transfer into noncancer-initiating cells
• K10plus-PPN: 1667590375

Abstract

Background: Cancer-initiating cell (CIC) exosomes (CIC-TEX) are suggested reprogramming Non-CIC. Mode of message transfer and engagement of CIC-markers being disputed, we elaborated the impact of CD44v6 and Tspan8 on the response of Non-CIC. Methods: Non-metastasizing CD44v6- and Tspan8-knockdown (kd) pancreatic cancer cells served as Non-CIC. CIC-TEX coculture-induced changes were evaluated by deep-sequencing and functional assays. Tumor progression was surveyed during in vivo CIC-TEX treatment. Results: Deep-sequencing of CIC-TEX-cocultured CD44v6kd-Non-CIC revealed pronounced mRNA changes in signaling, transport, transcription and translation; altered miRNA affected metabolism, signaling and transcription. CIC-TEX coculture-induced changes in Tspan8kd-Non-CIC mostly relied on CIC-TEX-Tspan8 being required for targeting. CIC-TEX transfer supported apoptosis resistance and significantly promoted epithelial mesenchymal transition, migration, invasion and (lymph)angiogenesis of the kd Non-CIC in vitro and in vivo, deep-sequencing allowing individual mRNA and miRNA assignment to altered functions. Importantly, CIC-TEX act as a hub, initiated by CD44v6-dependent RTK, GPCR and integrin activation and involving CD44v6-assisted transcription and RNA processing. Accordingly, a kinase inhibitor hampered CIC-TEX-fostered tumor progression, which was backed by an anti-Tspan8 blockade of CIC-TEX binding. Conclusions: This in depth report on the in vitro and in vivo impact of CIC-TEX on CD44v6kd and Tspan8kd Non-CIC unravels hub CIC-TEX activity, highlighting a prominent contribution of the CIC-markers CD44v6 to signaling cascade activation, transcription, translation and miRNA processing in Non-CIC and of Tspan8 to CIC-TEX targeting. Blocking CIC-TEX binding/uptake and uptake-initiated target cell activation significantly mitigated the deleterious CIC-TEX impact on CD44v6kd and Tspan8kd Non-CIC.