Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells

• Verfasst von: Eichhorn, Tolga [VerfasserIn]
• Verfasst von: Wendler, Astrid [VerfasserIn]
• Verfasst von: Krauth-Siegel, Renate [VerfasserIn]
• Titel: Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells
• Verf.angabe: T. Eichhorn, S. Schloissnig, B. Hahn, A. Wendler, Rolf Mertens, W.D. Lehmann, R.L. Krauth-Siegel and T. Efferth
• E-Jahr: 2012
• Jahr: 06 Feb 2012
• Umfang: 8 S.
• Fussnoten: Gesehen am 25.06.2019
• Titel Quelle: Enthalten in: Molecular BioSystems
• Ort Quelle: Cambridge : Royal Society of Chemistry, 2005
• Jahr Quelle: 2012
• Band/Heft Quelle: 8(2012), 4, Seite 1311-1318
• ISSN Quelle: 1742-2051
• DOI: doi:10.1039/C2MB05437J
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1667920650

Abstract

Determining interacting cellular partners of drugs by chemical proteomic techniques is complex and tedious. Most approaches rely on activity-based probe profiling and compound-centric chemical proteomics. The anti-malarial artemisinin also exerts profound anti-cancer activity, but the mechanisms of action are incompletely understood. In the present investigation, we present a novel approach to identify artemisinin-interacting target proteins. Our approach overcomes usual problems in traditional fishing procedures, because the drug was attached to a surface without further chemical modification. The proteins identified effect among others, cell cycle arrest, apoptosis, inhibition of angiogenesis, disruption of cell migration, and modulation of nuclear receptor responsiveness. Furthermore, a bioinformatic approach confirmed experimentally identified proteins and suggested a large number of other interacting proteins. Theoretically predicted interaction partners may serve as a starting point to complete the whole set of proteins binding artemisinin.