miR-615-5p is restrictedly expressed in cirrhotic and cancerous liver tissues and its overexpression alleviates the tumorigenic effects in hepatocellular carcinoma

• Verfasst von: El Tayebi, Hend M. [VerfasserIn]
• Verfasst von: Breuhahn, Kai [VerfasserIn]
• Titel: miR-615-5p is restrictedly expressed in cirrhotic and cancerous liver tissues and its overexpression alleviates the tumorigenic effects in hepatocellular carcinoma
• Verf.angabe: H.M. El Tayebi, K.A. Hosny, G. Esmat, K. Breuhahn, Ahmed Ihab Abdelaziz
• E-Jahr: 2012
• Jahr: 20 July 2012
• Umfang: 8 S.
• Fussnoten: Gesehen am 27.06.2019
• Titel Quelle: Enthalten in: Vereinigung der Europäischen Biochemischen GesellschaftenFEBS letters
• Ort Quelle: Chichester : Wiley, 1968
• Jahr Quelle: 2012
• Band/Heft Quelle: 586(2012), 19, Seite 3309-3316
• ISSN Quelle: 1873-3468
• DOI: doi:10.1016/j.febslet.2012.06.054
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Heptocellular carcinoma
• Sach-SW: microRNA-615-5p
• Sach-SW: Migration
• Sach-SW: Proliferation
• K10plus-PPN: 1668058936

Abstract

microRNAs aberrant behavior in heptocellular carcinoma (HCC) plays a major role in HCC pathogenesis. miR-615-5p expression has never been evaluated in HCC. We showed that miR-615-5p was preferentially expressed in HCC, cirrhotic liver tissues and HCC cell lines, but undetected in normal livers. Forced miR-615-5p expression in HCC cell lines led to significant decrease in cell growth and migration. In-silico predication revealed insulin-like growth factor-II (IGF-II) as a potential downstream target for miR-615-5p. Forcing the expression of miR-615-5p showed downregulation of IGF-II mRNA, as well as inhibition of the luciferase activity in a luciferase reporter vector harboring the IGF-II-3′UTR target sequence. miR-615-5p acts as tumor-suppressor in HCC through targeting IGF-II.