Status: Bibliographieeintrag
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| Verfasst von: | Warttinger, Ulrich [VerfasserIn]  |
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| | Giese, Christina [VerfasserIn] |
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| | Harenberg, Job [VerfasserIn] |
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| | Holmer, Erik [VerfasserIn] |
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| | Krämer, Roland [VerfasserIn] |
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| Titel: | A fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma |
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| Verf.angabe: | U. Warttinger, C. Giese, J. Harenberg, Erik Holmer, Roland Krämer |
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| E-Jahr: | 2016 |
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| Jahr: | 21 September 2016 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 03.07.2019 |
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| Titel Quelle: | Enthalten in: Analytical and bioanalytical chemistry |
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| Ort Quelle: | Berlin : Springer, 2002 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 408(2016), 28, Seite 8241-8251 |
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| ISSN Quelle: | 1618-2650 |
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| Abstract: | Heparins are widely used anticoagulant drugs. The current monitoring practice for heparin in plasma, such as the chromogenic anti-factor Xa assay, relies on heparin-triggered activation of antithrombin, an inhibitor of coagulation proteases. Such assays are not applicable to the detection of non-anticoagulant heparins, an emerging class of drug candidates for therapeutic applications unrelated to anticlotting activity. This study describes the application of a commercially available fluorescent probe assay (Heparin Red) for the direct and sensitive detection of the “chemical” heparin in plasma, independent of any anticoagulant activity. The quantification range is about 0-5 μg/mL for both unfractionated heparin (corresponding to 0-1 IU/mL) and the low molecular weight heparin enoxaparin. The Heparin Red assay is of particular value for the quantification of non-anticoagulant heparins, as exemplified by the low molecular weight heparin derivative tafoxiparin and a N-desulfated-N-reacetylated heparin. Heparin octa- and decasaccharides are also detected. Open image in new window Graphical abstract Heparin quantification in plasma by mixing the sample with the Heparin Red reagent and fluorescence readout |
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| DOI: | doi:10.1007/s00216-016-9940-y |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/s00216-016-9940-y |
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| | DOI: https://doi.org/10.1007/s00216-016-9940-y |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Assay |
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| | Fluorescent |
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| | Heparin Red |
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| | Non-anticoagulant heparins |
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| | Plasma |
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| | Tafoxiparin |
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| K10plus-PPN: | 166843282X |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma / Warttinger, Ulrich [VerfasserIn]; 21 September 2016 (Online-Ressource)
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