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| Verfasst von: | Herrmann, Anne-Kathrin [VerfasserIn]  |
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| | Kienle, Eike [VerfasserIn] |
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| | Große, Stefanie [VerfasserIn] |
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| | El Andari, Jihad [VerfasserIn] |
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| | Botta, Julia [VerfasserIn] |
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| | Schürmann, Nina [VerfasserIn] |
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| | Wiedtke, Ellen [VerfasserIn] |
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| | Niopek, Dominik [VerfasserIn] |
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| | Grimm, Dirk [VerfasserIn] |
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| Titel: | A robust and all-inclusive pipeline for shuffling of adeno-associated viruses |
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| Verf.angabe: | Anne-Kathrin Herrmann, Christian Bender, Eike Kienle, Stefanie Grosse, Jihad El Andari, Julia Botta, Nina Schürmann, Ellen Wiedtke, Dominik Niopek, and Dirk Grimm |
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| Jahr: | 2019 |
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| Jahr des Originals: | 2018 |
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| Umfang: | 13 S. |
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| Fussnoten: | Published: December 4, 2018 ; Gesehen am 05.07.2019 |
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| Titel Quelle: | Enthalten in: American Chemical SocietyACS synthetic biology |
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| Ort Quelle: | Washington, DC : ACS, 2012 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 8(2019), 1, Seite 194-206 |
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| ISSN Quelle: | 2161-5063 |
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| Abstract: | Adeno-associated viruses (AAV) are attractive templates for engineering of synthetic gene delivery vectors. A particularly powerful technology for breeding of novel vectors with improved properties is DNA family shuffling, i.e., generation of chimeric capsids by homology-driven DNA recombination. Here, to make AAV DNA shuffling available to a wider community, we present a robust experimental and bioinformatical pipeline comprising: (i) standardized and partially codon-optimized plasmids carrying 12 different AAV capsid genes; (ii) a scalable protocol including troubleshooting guide for viral library production; and (iii) the freely available software SALANTO for comprehensive analysis of chimeric AAV DNA and protein sequences. Moreover, we describe a set of 12 premade and ready-to-use AAV libraries. Finally, we demonstrate the usefulness of DNA barcoding technology to trace AAV capsid libraries within a complex mixture. Our protocols and resources facilitate the implementation and tailoring of AAV evolution technology in any laboratory interested in customized viral gene transfer. |
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| DOI: | doi:10.1021/acssynbio.8b00373 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1021/acssynbio.8b00373 |
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| | DOI: https://doi.org/10.1021/acssynbio.8b00373 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1668710501 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ robust and all-inclusive pipeline for shuffling of adeno-associated viruses / Herrmann, Anne-Kathrin [VerfasserIn]; 2019 (Online-Ressource)
