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| Verfasst von: | Fellenberg, Jörg [VerfasserIn]  |
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| | Sähr, Heiner [VerfasserIn] |
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| | Mancarella, D. [VerfasserIn] |
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| | Plass, C. [VerfasserIn] |
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| | Lindroth, A. M. [VerfasserIn] |
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| | Westhauser, Fabian [VerfasserIn] |
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| | Lehner, Burkhard [VerfasserIn] |
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| | Ewerbeck, Volker [VerfasserIn] |
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| Titel: | Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells |
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| Verf.angabe: | J. Fellenberg, H. Sähr, D. Mancarella, C. Plass, A.M. Lindroth, F. Westhauser, B. Lehner, V. Ewerbeck |
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| E-Jahr: | 2019 |
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| Jahr: | 8 February 2019 |
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| Umfang: | 9 S. |
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| Teil: | volume:448 |
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| | year:2019 |
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| | pages:61-69 |
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| | extent:9 |
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| Fussnoten: | Gesehen am 09.07.2019 |
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| Titel Quelle: | Enthalten in: Cancer letters |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1975 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 448(2019), Seite 61-69 |
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| ISSN Quelle: | 1872-7980 |
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| Abstract: | Giant cell tumors of bone (GCTB) are semi-malignant tumors associated with extensive osteolytic defects and massive bone destructions. They display a locally aggressive behavior and a very high recurrence rate. Recently, a single mutation has been identified in GCTB affecting the H3F3A gene coding for the histone variant H3.3 (H3.3-G34W). The aim of this study was to investigate whether H3.3-G34W is sufficient to drive tumorigenesis in GCTB. Initially, we confirmed the high frequency of this mutation in 94% of 84 analyzed tissue samples. Using a siRNA based approach we could selectively knockdown H3.3-G34W in primary neoplastic stromal cells isolated from tumor tissue (GCTSC). H3.3-G34W knockdown caused a significant inhibition of cell proliferation, migration and colony formation capacity in vitro. Xenotransplantation of GCTSCs onto the chorioallantoic membrane of fertilized chicken eggs further demonstrated a significant impact of H3.3-G34W knockdown on tumor engraftment and growth in vivo. Our data indicate that H3.3-G34W is sufficient to drive tumorigenesis in GCTB. Apart from the application of H3.3-G34W screening as diagnostic tool, our data suggest that H3.3-G4W represents a promising target for the development of new GCTB therapies. |
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| DOI: | doi:10.1016/j.canlet.2019.02.001 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.canlet.2019.02.001 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0304383519300631 |
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| | DOI: https://doi.org/10.1016/j.canlet.2019.02.001 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | CAM assay |
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| | Giant cell tumor of bone |
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| | H3.3-G34W |
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| | Histone |
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| | Neoplastic transformation |
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| K10plus-PPN: | 1668836467 |
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| Verknüpfungen: | → Zeitschrift |
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Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells / Fellenberg, Jörg [VerfasserIn]; 8 February 2019 (Online-Ressource)
