Cell type-dependent integrin distribution in adhesion and migration responses on protein-coated microgrooved substrates

• Verfasst von: Sales Ramos, Adrià [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Cell type-dependent integrin distribution in adhesion and migration responses on protein-coated microgrooved substrates
• Verf.angabe: Adria Sales, Karen Ende, Jennifer Diemer, Adriana R. Kyvik, Jaume Veciana, Imma Ratera, Ralf Kemkemer, Joachim P. Spatz, and Judith Guasch
• E-Jahr: 2019
• Jahr: 22 January 2019
• Umfang: 10 S.
• Fussnoten: Gesehen am 11.07.2019
• Titel Quelle: Enthalten in: ACS omega
• Ort Quelle: Washington, DC : ACS Publications, 2016
• Jahr Quelle: 2019
• Band/Heft Quelle: 4(2019), 1, Seite 1791-1800
• ISSN Quelle: 2470-1343
• DOI: doi:10.1021/acsomega.8b03608
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1669031004

Abstract

Although integrins are responsible for the interaction of cells with their environment, e.g., the extracellular matrix or artificial substrates, there is still a lack of knowledge about their role in cell adhesion and migration on protein-coated substrates with microtopography. Understanding such interactions could lead to new applications in e.g., medical implants as well as shed light on processes such as embryonic development, angiogenesis, wound healing, and tumor progression. In this work, the influence of surface topography and chemistry on αvβ3 and α5β1 integrin-mediated cell adhesion and migration of healthy and malignant human cell types (human coronary artery endothelial cells, human osteosarcoma cells, and human skin fibroblasts cells) was studied, using microgrooved and flat substrates covered by two different extracellular proteins, fibronectin and vitronectin. Although some general behaviors can be observed, cell migration (speed, directionality, and persistence time) and morphological adaptation (cell area, aspect ratio, and circularity) of cells on protein-coated microgrooved substrates are mainly dependent on the cell type and its specific integrin expression.