Impact of c-MYC expression on proliferation, differentiation, and risk of neoplastic transformation of human mesenchymal stromal cells

• Verfasst von: Melnik, Svitlana [VerfasserIn]
• Verfasst von: Boeuf, Stephane [VerfasserIn]
• Verfasst von: Richter, Wiltrud [VerfasserIn]
• Titel: Impact of c-MYC expression on proliferation, differentiation, and risk of neoplastic transformation of human mesenchymal stromal cells
• Verf.angabe: Svitlana Melnik, Nadine Werth, Stephane Boeuf, Eva-Maria Hahn, Tobias Gotterbarm, Martina Anton and Wiltrud Richter
• E-Jahr: 2019
• Jahr: 5 March 2019
• Umfang: 18 S.
• Teil: volume:10
• Teil: year:2019
• Teil: elocationid:73
• Teil: pages:1-18
• Teil: extent:18
• Fussnoten: Gesehen am 15.07.2019
• Titel Quelle: Enthalten in: Stem cell research & therapy
• Ort Quelle: London : BioMed Central, 2010
• Jahr Quelle: 2019
• Band/Heft Quelle: 10(2019), Artikel-ID 73, Seite 1-18
• ISSN Quelle: 1757-6512
• DOI: doi:10.1186/s13287-019-1187-z
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1669145484
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Mesenchymal stromal cells isolated from bone marrow (MSC) represent an attractive source of adult stem cells for regenerative medicine. However, thorough research is required into their clinical application safety issues concerning a risk of potential neoplastic degeneration in a process of MSC propagation in cell culture for therapeutic applications. Expansion protocols could preselect MSC with elevated levels of growth-promoting transcription factors with oncogenic potential, such as c-MYC. We addressed the question whether c-MYC expression affects the growth and differentiation potential of human MSC upon extensive passaging in cell culture and assessed a risk of tumorigenic transformation caused by MSC overexpressing c-MYC in vivo.