Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Scherer, Daniel [VerfasserIn]   i
 Seyler, Claudia [VerfasserIn]   i
 Xynogalos, Panagiotis [VerfasserIn]   i
 Scholz, Eberhard P. [VerfasserIn]   i
 Thomas, Dierk [VerfasserIn]   i
 Backs, Johannes [VerfasserIn]   i
 Andrassy, Martin [VerfasserIn]   i
 Völkers, Mirko [VerfasserIn]   i
 Karle, Christoph [VerfasserIn]   i
 Katus, Hugo [VerfasserIn]   i
 Zitron, Edgar [VerfasserIn]   i
Titel:Inhibition of cardiac Kir current (IK1) by protein kinase C critically depends on PKCβ and Kir2.2
Verf.angabe:Daniel Scherer, Claudia Seyler, Panagiotis Xynogalos, Eberhard P. Scholz, Dierk Thomas, Johannes Backs, Martin Andrassy, Mirko Völkers, Christoph A. Karle, Hugo A. Katus, Edgar Zitron
E-Jahr:2016
Jahr:May 23, 2016
Umfang:12 S.
Fussnoten:Im Titel ist "K1" in (IK1) tiefgestellt ; Gesehen am 18.07.2019
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2016
Band/Heft Quelle:11(2016,5) Artikel-Nummer e0156181
ISSN Quelle:1932-6203
Abstract:Background Cardiac inwardly rectifying Kir current (IK1) mediates terminal repolarisation and is critical for the stabilization of the diastolic membrane potential. Its predominant molecular basis in mammalian ventricle is heterotetrameric assembly of Kir2.1 and Kir2.2 channel subunits. It has been shown that PKC inhibition of IK1 promotes focal ventricular ectopy. However, the underlying molecular mechanism has not been fully elucidated to date. Methods and Results In the Xenopus oocyte expression system, we observed a pronounced PKC-induced inhibition of Kir2.2 but not Kir2.1 currents. The PKC regulation of Kir2.2 could be reproduced by an activator of conventional PKC isoforms and antagonized by pharmacological inhibition of PKCβ. In isolated ventricular cardiomyocytes (rat, mouse), pharmacological activation of conventional PKC isoforms induced a pronounced inhibition of IK1. The PKC effect in rat ventricular cardiomyocytes was markedly attenuated following co-application of a small molecule inhibitor of PKCβ. Underlining the critical role of PKCβ, the PKC-induced inhibition of IK1 was absent in homozygous PKCβ knockout-mice. After heterologous expression of Kir2.1-Kir2.2 concatemers in Xenopus oocytes, heteromeric Kir2.1/Kir2.2 currents were also inhibited following activation of PKC. Conclusion We conclude that inhibition of cardiac IK1 by PKC critically depends on the PKCβ isoform and Kir2.2 subunits. This regulation represents a potential novel target for the antiarrhythmic therapy of focal ventricular arrhythmias.
DOI:doi:10.1371/journal.pone.0156181
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0156181
 Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0156181
 DOI: https://doi.org/10.1371/journal.pone.0156181
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Arrhythmia
 Drug regulation
 Membrane potential
 Phospholipases
 Protein kinases
 Signal inhibition
 Tachycardia
 Xenopus oocytes
K10plus-PPN:1669420299
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68409828   QR-Code
zum Seitenanfang