| |  Online-Ressource |
|---|
| Verfasst von: | Dal Magro, Christina [VerfasserIn]  |
|---|
| | Keller, Patrick [VerfasserIn] |
|---|
| | Jacobs, Anja [VerfasserIn] |
|---|
| | Dieterich, Christoph [VerfasserIn] |
|---|
| Titel: | A vastly increased chemical variety of RNA modifications containing a thioacetal structure |
|---|
| Verf.angabe: | Christina Dal Magro, Patrick Keller, Annika Kotter, Stephan Werner, Victor Duarte, Virginie Marchand, Michael Ignarski, Anja Freiwald, Roman-Ulrich Müller, Christoph Dieterich, Yuri Motorin, Falk Butter, Mohamed Atta, Mark Helm |
|---|
| E-Jahr: | 2018 |
|---|
| Jahr: | 06 April 2018 |
|---|
| Umfang: | 5 S. |
|---|
| Fussnoten: | Gesehen am 22.07.2019 |
|---|
| Titel Quelle: | Enthalten in: Angewandte Chemie / International edition |
|---|
| Ort Quelle: | Weinheim : Wiley-VCH, 1962 |
|---|
| Jahr Quelle: | 2018 |
|---|
| Band/Heft Quelle: | 57(2018), 26, Seite 7893-7897 |
|---|
| ISSN Quelle: | 1521-3773 |
|---|
| Abstract: | Recently discovered new chemical entities in RNA modifications have involved surprising functional groups that enlarge the chemical space of RNA. Using LC-MS, we found over 100 signals of RNA constituents that contained a ribose moiety in tRNAs from E. coli. Feeding experiments with variegated stable isotope labeled compounds identified 37 compounds that are new structures of RNA modifications. One structure was elucidated by deuterium exchange and high-resolution mass spectrometry. The structure of msms2i6A (2-methylthiomethylenethio-N6-isopentenyl-adenosine) was confirmed by methione-D3 feeding experiments and by synthesis of the nucleobase. The msms2i6A contains a thioacetal, shown in vitro to be biosynthetically derived from ms2i6A by the radical-SAM enzyme MiaB. This enzyme performs thiomethylation, forming ms2i6A from i6A in a first turnover. The new thioacetal is formed by a second turnover. Along with the pool of 36 new modifications, this work describes a new layer of RNA modification chemistry. |
|---|
| DOI: | doi:10.1002/anie.201713188 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/anie.201713188 |
|---|
| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201713188 |
|---|
| | DOI: https://doi.org/10.1002/anie.201713188 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | isotope labelling |
|---|
| | LC-MS |
|---|
| | radical-SAM enzymes |
|---|
| | RNA modifications |
|---|
| | thioacetals |
|---|
| K10plus-PPN: | 1669553485 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
¬A¬ vastly increased chemical variety of RNA modifications containing a thioacetal structure / Dal Magro, Christina [VerfasserIn]; 06 April 2018 (Online-Ressource)
