A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka

• Verfasst von: Herpin, Amaury [VerfasserIn]
• Verfasst von: Ettwiller, Laurence [VerfasserIn]
• Verfasst von: Dolle, Dirk-Dominik [VerfasserIn]
• Titel: A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka
• Verf.angabe: Amaury Herpin, Cornelia Schmidt, Susanne Kneitz, Clara Gobé, Martina Regensburger, Aurélie Le Cam, Jérome Montfort, Mateus C. Adolfi, Christina Lillesaar, Dagmar Wilhelm, Michael Kraeussling, Brigitte Mourot, Béatrice Porcon, Maëlle Pannetier, Eric Pailhoux, Laurence Ettwiller, Dirk Dolle, Yann Guiguen, Manfred Schartl
• E-Jahr: 2019
• Jahr: April 4, 2019
• Umfang: 29 S.
• Fussnoten: Gesehen am 23.07.2019
• Titel Quelle: Enthalten in: Public Library of SciencePLoS biology
• Ort Quelle: Lawrence, KS : PLoS, 2003
• Jahr Quelle: 2019
• Band/Heft Quelle: 17(2019,4) Artikel-Nummer e3000185, 29 Seiten
• ISSN Quelle: 1545-7885
• DOI: doi:10.1371/journal.pbio.3000185
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Embryos
• Sach-SW: Gene expression
• Sach-SW: Germ cells
• Sach-SW: Gonads
• Sach-SW: Messenger RNA
• Sach-SW: Oryzias
• Sach-SW: Sequence motif analysis
• Sach-SW: Untranslated regions
• K10plus-PPN: 1669621944

Abstract

Dmrt1 is a highly conserved transcription factor, which is critically involved in regulation of gonad development of vertebrates. In medaka, a duplicate of dmrt1—acting as master sex-determining gene—has a tightly timely and spatially controlled gonadal expression pattern. In addition to transcriptional regulation, a sequence motif in the 3′ UTR (D3U-box) mediates transcript stability of dmrt1 mRNAs from medaka and other vertebrates. We show here that in medaka, two RNA-binding proteins with antagonizing properties target this D3U-box, promoting either RNA stabilization in germ cells or degradation in the soma. The D3U-box is also conserved in other germ-cell transcripts, making them responsive to the same RNA binding proteins. The evolutionary conservation of the D3U-box motif within dmrt1 genes of metazoans—together with preserved expression patterns of the targeting RNA binding proteins in subsets of germ cells—suggest that this new mechanism for controlling RNA stability is not restricted to fishes but might also apply to other vertebrates.