| |  Online-Ressource |
|---|
| Verfasst von: | Jahnke, Kevin [VerfasserIn]  |
|---|
| | Weiss, Marian [VerfasserIn] |
|---|
| | Frey, Christoph [VerfasserIn] |
|---|
| | Antona, Silvia [VerfasserIn] |
|---|
| | Janiesch, Jan-Willi [VerfasserIn] |
|---|
| | Platzman, Ilia [VerfasserIn] |
|---|
| | Göpfrich, Kerstin [VerfasserIn] |
|---|
| | Spatz, Joachim P. [VerfasserIn] |
|---|
| Titel: | Programmable functionalization of surfactant-stabilized microfluidic droplets via DNA-tags |
|---|
| Verf.angabe: | Kevin Jahnke, Marian Weiss, Christoph Frey, Silvia Antona, Jan-Willi Janiesch, Ilia Platzman, Kerstin Göpfrich, and Joachim P. Spatz |
|---|
| E-Jahr: | 2019 |
|---|
| Jahr: | April 8, 2019 |
|---|
| Umfang: | 8 S. |
|---|
| Fussnoten: | Gesehen am 12.08.2019 |
|---|
| Titel Quelle: | Enthalten in: Advanced functional materials |
|---|
| Ort Quelle: | Weinheim : Wiley-VCH, 2001 |
|---|
| Jahr Quelle: | 2019 |
|---|
| Band/Heft Quelle: | 29(2019), 23, Artikel-ID 1808647, Seite 1-8 |
|---|
| ISSN Quelle: | 1616-3028 |
|---|
| Abstract: | Droplet-based microfluidics has emerged as a powerful tool in synthetic biology. For many applications, chemical functionalization of the droplets is a key process. Therefore, a straightforward and broadly applicable approach is developed to functionalize the inner periphery of microfluidic droplets with diverse reactive groups and components. Instead of covalent modification of the droplet-stabilizing surfactants, this method relies on cholesterol-tagged DNA that self-assembles at the droplet periphery. The cholesterol-tagged DNA serves as an attachment handle for the recruitment of complementary DNA. The complementary DNA can carry diverse functional groups. We exemplify our method by demonstrating the attachment of amine groups, DNA nanostructures, microspheres, a minimal actin cortex, and leukemia cells to the droplet periphery. It is further shown that the DNA-mediated attachment to the droplet periphery is temperature-responsive and reversible. It is envisioned that droplet functionalization via DNA handles will help to tailor droplet interfaces for diverse applications—featuring programmable assembly, unique addressability, and stimuli-responsiveness. |
|---|
| DOI: | doi:10.1002/adfm.201808647 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1002/adfm.201808647 |
|---|
| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201808647 |
|---|
| | DOI: https://doi.org/10.1002/adfm.201808647 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | block copolymer surfactant |
|---|
| | cholesterol-tagged DNA |
|---|
| | DNA nanotechnology |
|---|
| | droplet-based microfluidics |
|---|
| | functionalization |
|---|
| K10plus-PPN: | 1671195868 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Programmable functionalization of surfactant-stabilized microfluidic droplets via DNA-tags / Jahnke, Kevin [VerfasserIn]; April 8, 2019 (Online-Ressource)
