Live cell imaging of cytoplasmic and nuclear Ca2+ dynamics in Arabidopsis roots

• Verfasst von: Krebs, Melanie [VerfasserIn]
• Verfasst von: Schumacher, Karin [VerfasserIn]
• Titel: Live cell imaging of cytoplasmic and nuclear Ca2+ dynamics in Arabidopsis roots
• Verf.angabe: Melanie Krebs, Karin Schumacher
• E-Jahr: 2013
• Jahr: 2013 Aug 1
• Umfang: 5 S.
• Fussnoten: Im Titel ist „2+“ hochgestellt ; Gesehen am 14.08.2019
• Titel Quelle: Enthalten in: Cold Spring Harbor protocols
• Ort Quelle: Cold Spring Harbor, NY, 2006
• Jahr Quelle: 2013
• Band/Heft Quelle: (2013), 8, Seite 776-780
• ISSN Quelle: 1559-6095
• DOI: doi:10.1101/pdb.prot073031
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Arabidopsis
• Sach-SW: Calcium
• Sach-SW: Calcium-Binding Proteins
• Sach-SW: Cations, Divalent
• Sach-SW: Cell Nucleus
• Sach-SW: Cytological Techniques
• Sach-SW: Cytoplasm
• Sach-SW: Fluorescent Dyes
• Sach-SW: Microscopy, Confocal
• Sach-SW: Plant Roots
• Sach-SW: Staining and Labeling
• Sach-SW: Transgenes
• K10plus-PPN: 1671389883

Abstract

Approaches for Ca(2+) measurement require data recording with high spatiotemporal resolution. This is achieved by combined usage of locally targeted genetically encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). This protocol provides instructions for setting up a CLSM-based experiment to measure cytosolic and nuclear Ca(2+) dynamics in Arabidopsis lines expressing Yellow Cameleon Ca(2+) indicators.