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Verfasst von:Brenner, Nicole [VerfasserIn]   i
 Pawlita, Michael [VerfasserIn]   i
Titel:Validation of multiplex serology detecting human herpesviruses 1-5
Verf.angabe:Nicole Brenner, Alexander J. Mentzer, Julia Butt, Angelika Michel, Kristina Prager, Johannes Brozy, Benedikt Weißbrich, Allison E. Aiello, Helen C.S. Meier, Judy Breuer, Rachael Almond, Naomi Allen, Michael Pawlita, Tim Waterboer
E-Jahr:2018
Jahr:December 27, 2018
Umfang:20 S.
Teil:volume:13
 year:2018
 number:12
 extent:20
Fussnoten:Gesehen am 15.08.2019
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2018
Band/Heft Quelle:13(2018,12) Artikel-Nummer e0209379, 20 Seiten
ISSN Quelle:1932-6203
Abstract:Human herpesviruses (HHV) cause a variety of clinically relevant conditions upon primary infection of typically young and immunocompetent hosts. Both primary infection and reactivation after latency can lead to more severe disease, such as encephalitis, congenital defects and cancer. Infections with HHV are also associated with cardiovascular and neurodegenerative disease. However, most of the associations are based on retrospective case-control analyses and well-powered prospective cohort studies are needed for assessing temporality and causality. To enable comprehensive investigations of HHV-related disease etiology in large prospective population-based cohort studies, we developed HHV Multiplex Serology. This methodology represents a low-cost, high-throughput technology that allows simultaneous measurement of specific antibodies against five HHV species: Herpes simplex viruses 1 and 2, Varicella zoster virus, Epstein-Barr virus, and Cytomegalovirus. The newly developed HHV species-specific (‘Monoplex’) assays were validated against established gold-standard reference assays. The specificity and sensitivity of the HHV species-specific Monoplex Serology assays ranged from 92.3% to 100.0% (median 97.4%) and 91.8% to 98.7% (median 96.6%), respectively. Concordance with reference assays was very high with kappa values ranging from 0.86 to 0.96 (median kappa 0.93). Multiplexing the Monoplex Serology assays resulted in no loss of performance and allows simultaneous detection of antibodies against the 5 HHV species in a high-throughput manner.
DOI:doi:10.1371/journal.pone.0209379
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0209379
 Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0209379
 DOI: https://doi.org/10.1371/journal.pone.0209379
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Serology
 Antibodies
 Antigens
 Enzyme-linked immunoassays
 Epstein-Barr virus
 Herpesviruses
 Kaposi's sarcoma-associated herpesvirus
 Sequence databases
K10plus-PPN:1671455444
Verknüpfungen:→ Zeitschrift

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