| |  Online-Ressource |
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| Verfasst von: | Silva-Vilches, Cinthia [VerfasserIn]  |
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| | Pletinckx, Katrien [VerfasserIn] |
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| | Lohnert, Miriam [VerfasserIn] |
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| | Pavlovic, Vladimir [VerfasserIn] |
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| | Ashour, Diyaaeldin [VerfasserIn] |
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| | John, Vini [VerfasserIn] |
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| | Vendelova, Emilia [VerfasserIn] |
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| | Kneitz, Susanne [VerfasserIn] |
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| | Zhou, Jie [VerfasserIn] |
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| | Chen, Rena [VerfasserIn] |
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| | Reinheckel, Thomas [VerfasserIn] |
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| | Müller, Thomas [VerfasserIn] |
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| | Bodem, Jochen [VerfasserIn] |
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| | Lutz, Manfred B. [VerfasserIn] |
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| Titel: | Low doses of cholera toxin and its mediator cAMP induce CTLA-2 secretion by dendritic cells to enhance regulatory T cell conversion |
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| Verf.angabe: | Cinthia Silva-Vilches, Katrien Pletinckx, Miriam Lohnert, Vladimir Pavlovic, Diyaaeldin Ashour, Vini John, Emilia Vendelova, Susanne Kneitz, Jie Zhou, Rena Chen, Thomas Reinheckel, Thomas D. Mueller, Jochen Bodem, Manfred B. Lutz |
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| E-Jahr: | 2017 |
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| Jahr: | July 31, 2017 |
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| Umfang: | 20 S. |
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| Fussnoten: | Gesehen am 19.08.2019 |
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| Titel Quelle: | Enthalten in: PLOS ONE |
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| Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
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| Jahr Quelle: | 2017 |
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| Band/Heft Quelle: | 12(2017,7) Artikel-Nummer e0178114, 20 Seiten |
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| ISSN Quelle: | 1932-6203 |
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| Abstract: | Immature or semi-mature dendritic cells (DCs) represent tolerogenic maturation stages that can convert naive T cells into Foxp3+ induced regulatory T cells (iTreg). Here we found that murine bone marrow-derived DCs (BM-DCs) treated with cholera toxin (CT) matured by up-regulating MHC-II and costimulatory molecules using either high or low doses of CT (CThi, CTlo) or with cAMP, a known mediator CT signals. However, all three conditions also induced mRNA of both isoforms of the tolerogenic molecule cytotoxic T lymphocyte antigen 2 (CTLA-2α and CTLA-2β). Only DCs matured under CThi conditions secreted IL-1β, IL-6 and IL-23 leading to the instruction of Th17 cell polarization. In contrast, CTlo- or cAMP-DCs resembled semi-mature DCs and enhanced TGF-β-dependent Foxp3+ iTreg conversion. iTreg conversion could be reduced using siRNA blocking of CTLA-2 and reversely, addition of recombinant CTLA-2α increased iTreg conversion in vitro. Injection of CTlo- or cAMP-DCs exerted MOG peptide-specific protective effects in experimental autoimmune encephalomyelitis (EAE) by inducing Foxp3+ Tregs and reducing Th17 responses. Together, we identified CTLA-2 production by DCs as a novel tolerogenic mediator of TGF-β-mediated iTreg induction in vitro and in vivo. The CT-induced and cAMP-mediated up-regulation of CTLA-2 also may point to a novel immune evasion mechanism of Vibrio cholerae. |
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| DOI: | doi:10.1371/journal.pone.0178114 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1371/journal.pone.0178114 |
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| | Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0178114 |
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| | DOI: https://doi.org/10.1371/journal.pone.0178114 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cell differentiation |
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| | Cholera |
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| | Cytokines |
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| | Immune evasion |
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| | Regulatory T cells |
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| | Small interfering RNAs |
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| | T cells |
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| | Toxins |
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| K10plus-PPN: | 167160590X |
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| Verknüpfungen: | → Zeitschrift |
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Low doses of cholera toxin and its mediator cAMP induce CTLA-2 secretion by dendritic cells to enhance regulatory T cell conversion / Silva-Vilches, Cinthia [VerfasserIn]; July 31, 2017 (Online-Ressource)
