Charge-controlled microfluidic formation of lipid-based single- and multicompartment systems

• Verfasst von: Haller, Barbara [VerfasserIn]
• Verfasst von: Göpfrich, Kerstin [VerfasserIn]
• Verfasst von: Schröter, Martin [VerfasserIn]
• Verfasst von: Janiesch, Jan-Willi [VerfasserIn]
• Verfasst von: Platzman, Ilia [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Charge-controlled microfluidic formation of lipid-based single- and multicompartment systems
• Verf.angabe: Barbara Haller, Kerstin Göpfrich, Martin Schröter, Jan-Willi Janiesch, Ilia Platzman, and Joachim P. Spatz
• E-Jahr: 2018
• Jahr: 26 Jul 2018
• Umfang: 10 S.
• Fussnoten: Gesehen am 21.08.2019
• Titel Quelle: Enthalten in: Lab on a chip
• Ort Quelle: Cambridge : RSC, 2001
• Jahr Quelle: 2018
• Band/Heft Quelle: 18(2018), 17, Seite 2665-2674
• ISSN Quelle: 1473-0189
• DOI: doi:10.1039/C8LC00582F
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1671732928

Abstract

In this manuscript, we introduce a simple, off-the-shelf approach for the on-demand creation of giant unilamellar vesicles (GUVs) or multicompartment synthetic cell model systems in a high-throughput manner. To achieve this, we use microfluidics to encapsulate small unilamellar vesicles in block-copolymer surfactant-stabilized water-in-oil droplets. By tuning the charge of the inner droplet interface, adsorption of lipids can be either inhibited, leading to multicompartment systems, or induced, leading to the formation of droplet-stabilized GUVs. To control the charge density, we formed droplets using different molar ratios of an uncharged PEG-based fluorosurfactant and a negatively-charged PFPE carboxylic acid fluorosurfactant (Krytox). We systematically studied the transition from a multicompartment system to 3D-supported lipid bilayers as a function of lipid charge and Krytox concentration using confocal fluorescence microscopy, cryo-scanning electron microscopy and interfacial tension measurements. Moreover, we demonstrate a simple method to release GUVs from the surfactant shell and the oil phase into a physiological buffer - providing a remarkably high-yield approach for GUV formation. This widely applicable microfluidics-based technology will increase the scope of GUVs as adaptable cell-like compartments in bottom-up synthetic biology applications and beyond.