A chromogenic and fluorogenic peptide substrate for the highly sensitive detection of proteases in biological matrices

• Verfasst von: Arian, Dumitru [VerfasserIn]
• Verfasst von: Harenberg, Job [VerfasserIn]
• Verfasst von: Krämer, Roland [VerfasserIn]
• Titel: A chromogenic and fluorogenic peptide substrate for the highly sensitive detection of proteases in biological matrices
• Verf.angabe: Dumitru Arian, Job Harenberg, and Roland Krämer
• E-Jahr: 2016
• Jahr: July 27, 2016
• Umfang: 8 S.
• Fussnoten: Gesehen am 23.08.2019
• Titel Quelle: Enthalten in: Journal of medicinal chemistry
• Ort Quelle: Washington, DC : ACS, 1959
• Jahr Quelle: 2016
• Band/Heft Quelle: 59(2016), 16, Seite 7576-7583
• ISSN Quelle: 1520-4804
• DOI: doi:10.1021/acs.jmedchem.6b00652
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 167192892X

Abstract

The synthesis and application of a novel type of chromogenic and fluorogenic substrate for protease detection is described. The outstanding performance of the tripeptide substrates is exemplified by specific fluorescence detection of thrombin and factor Xa at only 500 fM concentration. The substrate is also applicable to the sensitive detection of the thrombin inhibitor dabigatran in human plasma and whole blood samples, highlighting its potential for a point-of-care test for instant monitoring the blood levels of this blockbuster anticoagulant drug in specific clinical situations.